Certain types of iron oxide nanoparticles are not suited to passively target inflammatory cells that infiltrate the brain in response to stroke.
Certain types of iron oxide nanoparticles are not suited to passively target inflammatory cells that infiltrate the brain in response to stroke.
复制标题
DOI：
10.1038/jcbfm.2013.22
复制
发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
在实验性卒中急性期静脉注射氧化铁纳米颗粒可以在缺血区产生信号强度变化。尽管有争议，但这归因于富含铁的血液巨噬细胞的渗透。纳米颗粒的特性使其最适合吞噬作用，这是一个有争议的问题，也是最相关的给药时间点。这两个问题都在本研究中得到了检验。建立小鼠大脑中动脉闭塞30 模型，进行脑成像实验。使用不同电荷和大小的氧化铁纳米颗粒，小鼠静脉注射300mmolFe/kg：超顺磁性氧化铁纳米颗粒(SPIO)、超小SPIO或非常小的SPIO。在大脑中动脉阻塞前7天、再灌流时或大脑中动脉阻塞后72 小时给药。有趣的是，在缺血的大脑中没有观察到的信号变化，这可以归因于铁。此外，尽管在肝脏和脾中有强烈的染色，但在脑或血白细胞中没有发现普鲁士蓝阳性细胞。这意味着为这项研究选择的纳米颗粒不会被血液传播的白细胞吞噬，也不会进入缺血小鼠的大脑。
Intravenous administration of iron oxide nanoparticles during the acute stage of experimental stroke can produce signal intensity changes in the ischemic region. This has been attributed, albeit controversially, to the infiltration of iron-laden blood-borne macrophages. The properties of nanoparticles that render them most suitable for phagocytosis is a matter of debate, as is the most relevant timepoint for administration. Both of these questions are examined in the present study. Imaging experiments were performed in mice with 30 minutes of middle cerebral artery occlusion (MCAO). Iron oxide nanoparticles with different charges and sizes were used, and mice received 300 μmol Fe/kg intravenously: either superparamagnetic iron oxide nanoparticles (SPIOs), ultrasmall SPIOs, or very small SPIOs. The particles were administered 7 days before MCAO, at the time of reperfusion, or 72 hours after MCAO. Interestingly, there was no observable signal change in the ischemic brains that could be attributed to iron. Furthermore, no Prussian blue-positive cells were found in the brains or blood leukocytes, despite intense staining in the livers and spleens. This implies that the nanoparticles selected for this study are not phagocytosed by blood-borne leukocytes and do not enter the ischemic mouse brain.
登录
查看更多内容
影响因子：
6.3
作者：
Denes, Adam;Vidyasagar, Rishma;Allan, Stuart M.
通讯作者：
Allan, Stuart M.
影响因子：
8.3
作者：
Desestret, Virginie;Brisset, Jean-Christophe;Wiart, Marlene
通讯作者：
Wiart, Marlene
影响因子：
8.3
作者：
Wiart, Marlene;Davoust, Nathalie;Berthezene, Yves
通讯作者：
Berthezene, Yves
影响因子：
2.9
作者：
Farr, Tracy D.;Seehafer, Joerg U.;Hoehn, Mathias
通讯作者：
Hoehn, Mathias
影响因子：
3.3
作者：
Rausch, M;Sauter, A;Rudin, M
通讯作者：
Rudin, M