Induction of luteinizing hormone receptors by adenosine 3',5'-monophosphate in cultured granulosa cells.
Induction of luteinizing hormone receptors by adenosine 3',5'-monophosphate in cultured granulosa cells.
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在培养的颗粒细胞中通过腺苷 3,5-一磷酸诱导黄体生成激素受体。
DOI：
10.1210/endo-111-4-1192
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
K. Catt
中科院分区：
文献类型：
--
作者：
M. Knecht;K. Catt
在垂体切除的植入己烯雌酚的大鼠卵巢的颗粒细胞中检查了外源性和内源性 cAMP 对 LH 受体的诱导。在 96 小时培养期间，FSH 刺激 cAMP 产生、蛋白质合成、孕酮形成和 LH 受体表达。 2.5-10 ng FSH 刺激 cAMP 积累和 LH 受体形成，并在 175 ng FSH 培养 72 小时时达到最大。培养 48 小时后黄体酮产量达到峰值，但需要 25 ng FSH 来启动类固醇生成，需要 375 ng 才能产生最大反应。在用 5 mM 8-溴-cAMP (8-Br-cAMP) 培养的细胞中，48 小时时孕酮的产生与 FSH 刺激的相似，LH 受体的形成是 FSH 刺激的三分之一。培养 48 小时后去除 8-BrcAMP 导致 96 小时时 LH 受体和孕酮产量下降，表明需要持续存在环核苷酸才能维持颗粒细胞功能。 LH 受体诱导...
The induction of LH receptors by exogenous and endogenous cAMP was examined in granulosa cells from ovaries of hypophysectomized diethylstilbestrol-implanted rats. During the 96-h culture period, FSH stimulated cAMP production, protein synthesis, progesterone formation, and the expression of LH receptors. cAMP accumulation and LH receptor formation were stimulated by 2.5–10 ng FSH, and were maximal at 72 h of culture with 175 ng FSH. Progesterone production peaked after 48 h of culture, but required 25 ng FSH to initiate steroidogenesis and 375 ng for the maximal response. In cells cultured with 5 mM 8-bromo-cAMP (8-Br-cAMP), progesterone production at 48 h was similar to that stimulated by FSH, and LH receptor formation was one third as much as with FSH. Removal of 8-BrcAMP after 48 h of culture caused a fall in LH receptors and progesterone production at 96 h, indicating that continual presence of the cyclic nucleotide was required for the maintenance of granulosa cell function. The LH receptors induced...
影响因子：
4.8
作者：
LaBarbera,AR;Ryan,RJ
通讯作者：
Ryan,RJ
DOI：
10.1016/s0021-9258(19)70116-1
发表时间：
1981-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Ascoli
通讯作者：
M. Ascoli
在猪颗粒细胞单层培养过程中，卵泡刺激素介导功能性黄体生成素/人绒毛膜促性腺激素受体的诱导。
影响因子：
4.8
作者：
May,JV;McCartyJr,K;ReichertJr,LE;Schomberg,DW
通讯作者：
Schomberg,DW