An undergraduate laboratory experiment with real-world applications: Utilizing templateless polymerase chain reaction and real-time polymerase chain reaction to test for SARS-CoV-2 RNA.
An undergraduate laboratory experiment with real-world applications: Utilizing templateless polymerase chain reaction and real-time polymerase chain reaction to test for SARS-CoV-2 RNA.
复制标题
DOI：
10.1002/bmb.21593
复制
发表时间：
2022-01
影响因子：
1.4
通讯作者：
Page, Shallee T.
中科院分区：
文献类型：
--
作者：
Crane, Julia;Page, Shallee T.
We present a course‐embedded undergraduate research module that involves real‐time polymerase chain reaction testing for the presence of SARS‐CoV‐2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real‐world assay development. The exercise is aimed at upper‐level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper‐level classes including molecular biology, biochemistry, biotechnology, or for independent research projects.
登录
查看更多内容
DOI：
10.1187/cbe.18-03-0036
发表时间：
2018-12
期刊：
CBE life sciences education
影响因子：
--
作者：
Gin LE;Rowland AA;Steinwand B;Bruno J;Corwin LA
通讯作者：
Corwin LA
影响因子：
19
作者：
Singanayagam, Anika;Patel, Monika;Gopal, Robin
通讯作者：
Gopal, Robin
影响因子：
1.9
作者：
Meza-Robles, Carmen;Barajas-Saucedo, Carlos E.;Delgado-Enciso, Ivan
通讯作者：
Delgado-Enciso, Ivan
影响因子：
3
作者：
Elkins, Kelly M.;Kadunc, Raelynn E.
通讯作者：
Kadunc, Raelynn E.
DOI：
10.1187/cbe.07-06-0039
发表时间：
2007-01-01
期刊：
CBE life sciences education
影响因子：
--
作者：
Lopatto, David
通讯作者：
Lopatto, David