Identification and Characterization of Wheat Yellow Striate Virus, a Novel Leafhopper-Transmitted Nucleorhabdovirus Infecting Wheat.
Identification and Characterization of Wheat Yellow Striate Virus, a Novel Leafhopper-Transmitted Nucleorhabdovirus Infecting Wheat.
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小麦黄纹病毒的鉴定和特征，一种新型叶蝉传播的核弹状病毒感染小麦。
DOI：
10.3389/fmicb.2018.00468
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang X
中科院分区：
文献类型：
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作者：
Liu Y;Du Z;Wang H;Zhang S;Cao M;Wang X
在中国发现了一种新的小麦病毒性病害。通过透射电镜观察，在小麦叶片细胞核内发现了长180 ~ 210 nm、宽35 ~ 40 nm的子弹状病毒颗粒。鉴定出一种假定的小麦侵染横纹病病毒，并将其暂定为小麦黄纹病毒（WYSV）。采用转录组测序和RACE分析方法，确定了小麦和叶蝉WYSV的全长核苷酸序列。WYSV的负义RNA基因组包含14486个核苷酸（nt）， 7个开放阅读框（orf）编码反义链上N-P-P3-M-P6-G-L顺序的推断蛋白。此外，WYSV基因组有一个76 nt的3 ‘先导RNA和一个258 nt的5 ’预告RNA， orf被保守的基因间序列分隔开。整个基因组序列与水稻黄矮病毒（RYSV-A和RYSV-B）两株基因组的核苷酸序列同源性分别为58.1%和57.7%。WYSV和RYSV-B的L蛋白氨基酸序列同源性最高，为63.8%，P6蛋白氨基酸序列同源性最低，为29.5%。系统发育分析确定WYSV是核habdovirus属的新成员。总的来说，这项研究提供了证据，表明WYSV可能是第一个被描述通过叶蝉传播感染小麦的核虫病毒。
A new wheat viral disease was found in China. Bullet-shaped viral particles within the nucleus of the infected wheat leave cells, which possessed 180–210 nm length and 35–40 nm width, were observed under transmission electron microscopy. A putative wheat-infecting rhabdovirus vectored by the leafhopper Psammotettix alienus was identified and tentatively named wheat yellow striate virus (WYSV). The full-length nucleotide sequence of WYSV was determined using transcriptome sequencing and RACE analysis of both wheat samples and leafhoppers P. alienus. The negative-sense RNA genome of WYSV contains 14,486 nucleotides (nt) and seven open reading frames (ORFs) encode deduced proteins in the order N-P-P3-M-P6-G-L on the antisense strand. In addition, WYSV genome has a 76-nt 3′ leader RNA and a 258-nt 5′ trailer, and the ORFs are separated by conserved intergenic sequences. The entire genome sequence shares 58.1 and 57.7% nucleotide sequence identity with two strains of rice yellow stunt virus (RYSV-A and RYSV-B) genomes, respectively. The highest amino acid sequence identity was 63.8% between the L proteins of the WYSV and RYSV-B, but the lowest was 29.5% between the P6 proteins of these viruses. Phylogenetic analysis firmly established WYSV as a new member of the genus Nucleorhabdovirus. Collectively, this study provided evidence that WYSV is likely the first nucleorhabdovirus described infecting wheat via leafhopper P. alienus transmission.
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影响因子：
2.7
作者：
Amarasinghe GK;Bào Y;Basler CF;Bavari S;Beer M;Bejerman N;Blasdell KR;Bochnowski A;Briese T;Bukreyev A;Calisher CH;Chandran K;Collins PL;Dietzgen RG;Dolnik O;Dürrwald R;Dye JM;Easton AJ;Ebihara H;Fang Q;Formenty P;Fouchier RAM;Ghedin E;Harding RM;Hewson R;Higgins CM;Hong J;Horie M;James AP;Jiāng D;Kobinger GP;Kondo H;Kurath G;Lamb RA;Lee B;Leroy EM;Li M;Maisner A;Mühlberger E;Netesov SV;Nowotny N;Patterson JL;Payne SL;Paweska JT;Pearson MN;Randall RE;Revill PA;Rima BK;Rota P;Rubbenstroth D;Schwemmle M;Smither SJ;Song Q;Stone DM;Takada A;Terregino C;Tesh RB;Tomonaga K;Tordo N;Towner JS;Vasilakis N;Volchkov VE;Wahl-Jensen V;Walker PJ;Wang B;Wang D;Wang F;Wang LF;Werren JH;Whitfield AE;Yan Z;Ye G;Kuhn JH
通讯作者：
Kuhn JH
影响因子：
1.6
作者：
Kundu, J. K.;Gadiou, S.;Cervena, G.
通讯作者：
Cervena, G.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
5
作者：
Dietzgen RG;Kondo H;Goodin MM;Kurath G;Vasilakis N
通讯作者：
Vasilakis N
影响因子：
14.8
作者：
通讯作者：
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