Exploring the synergy of radiative coupling and substrate undercut in arrayed gold nanodisks for economical, ultra-sensitive label-free biosensing.
Exploring the synergy of radiative coupling and substrate undercut in arrayed gold nanodisks for economical, ultra-sensitive label-free biosensing.
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DOI：
10.1109/jsen.2021.3111125
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发表时间：
2021
影响因子：
4.3
通讯作者：
Shih WC
中科院分区：
文献类型：
--
作者：
Misbah I;Ohannesian N;Qiao Y;Lin SH;Shih WC
我们报道了辐射耦合阵列金纳米盘在不可见衬底（AGNIS）作为一个具有成本效益的，高性能的纳米等离子体生物传感平台。通过衬底底切，纳米盘周围的电场分布已经恢复到好像纳米盘被单个介质包围，从而提供分析物对以其他方式掩埋的增强电场的可及性。AGNIS基板已制造晶圆级的纳米球光刻，而不需要昂贵的光刻。首次研究了辐射耦合和衬底底切协同作用下的LSPR蓝移行为，其结果是折射率灵敏度从207 nm/RIU增加到578 nm/RIU。协同作用还提高了对单层中性抗生物素蛋白-生物素结合的表面灵敏度，从7.4 nm到20.3 nm，中性抗生物素蛋白的检测限（LOD）为50 fM，这是迄今为止报道的关于该特异性表面结合反应的最佳无标记结果之一。作为一种潜在的癌症诊断应用，细胞外囊泡（如癌症和正常细胞分泌的外泌体）的LOD在112-600（外泌体/μL）范围内，这在许多临床应用中已经足够。使用CD 9、CD 63和CD 81抗体，无标记分析显示所有三种表面抗原在癌症来源的外泌体中的表达增加。这项工作首次证明，阵列辐射耦合和衬底底切的强大协同作用可以在可见光谱中实现经济，超灵敏的生物传感，其中高质量，低成本的硅检测器可随时用于护理点应用。
We report radiatively coupled arrayed gold nanodisks on invisible substrate (AGNIS) as a cost-effective, high-performance platform for nanoplasmonic biosensing. By substrate undercut, the electric field distribution around the nanodisks has been restored to as if the nanodisks were surrounded by a single medium, thereby provides analyte accessibility to otherwise buried enhanced electric field. The AGNIS substrate has been fabricated by wafer-scale nanosphere lithography without the need for costly lithography. The LSPR blue-shifting behavior synergistically contributed by radiative coupling and substrate undercut have been investigated for the first time, which culminates in a remarkable refractive index sensitivity increase from 207 nm/RIU to 578 nm/RIU. The synergy also improves surface sensitivity to monolayer neutravidin-biotin binding from 7.4 nm to 20.3 nm with the limit of detection (LOD) of neutravidin at 50 fM, which is among the best label-free results reported to date on this specific surface binding reaction. As a potential cancer diagnostic application, extracellular vesicles such as exosomes excreted by cancer and normal cells were measured with a LOD within 112–600 (exosomes/μL), which would be sufficient in many clinical applications. Using CD9, CD63, and CD81 antibodies, label-free profiling has shown increased expression of all three surface antigens in cancer-derived exosomes. This work demonstrates, for the first time, strong synergy of arrayed radiative coupling and substrate undercut can enable economical, ultrasensitive biosensing in the visible light spectrum where high-quality, low-cost silicon detectors are readily available for point-of-care applications.
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影响因子：
24
作者：
Marbán E
通讯作者：
Marbán E
影响因子：
10.8
作者：
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通讯作者：
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影响因子：
3.5
作者：
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影响因子：
10.8
作者：
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影响因子：
3.7
作者：
Hall, W. Paige;Ngatia, Salome N.;Van Duyne, Richard P.
通讯作者：
Van Duyne, Richard P.