Predicting Lyme Disease From Patients' Peripheral Blood Mononuclear Cells Profiled With RNA-Sequencing.
Predicting Lyme Disease From Patients' Peripheral Blood Mononuclear Cells Profiled With RNA-Sequencing.
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DOI：
10.3389/fimmu.2021.636289
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发表时间：
2021
影响因子：
7.3
通讯作者：
Ma'ayan A
中科院分区：
文献类型：
--
作者：
Clarke DJB;Rebman AW;Bailey A;Wojciechowicz ML;Jenkins SL;Evangelista JE;Danieletto M;Fan J;Eshoo MW;Mosel MR;Robinson W;Ramadoss N;Bobe J;Soloski MJ;Aucott JN;Ma'ayan A
Although widely prevalent, Lyme disease is still under-diagnosed and misunderstood. Here we followed 73 acute Lyme disease patients and uninfected controls over a period of a year. At each visit, RNA-sequencing was applied to profile patients' peripheral blood mononuclear cells in addition to extensive clinical phenotyping. Based on the projection of the RNA-seq data into lower dimensions, we observe that the cases are separated from controls, and almost all cases never return to cluster with the controls over time. Enrichment analysis of the differentially expressed genes between clusters identifies up-regulation of immune response genes. This observation is also supported by deconvolution analysis to identify the changes in cell type composition due to Lyme disease infection. Importantly, we developed several machine learning classifiers that attempt to perform various Lyme disease classifications. We show that Lyme patients can be distinguished from the controls as well as from COVID-19 patients, but classification was not successful in distinguishing those patients with early Lyme disease cases that would advance to develop post-treatment persistent symptoms.
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影响因子：
3.7
作者：
Adrion ER;Aucott J;Lemke KW;Weiner JP
通讯作者：
Weiner JP
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3
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Ma'ayan A
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Kuleshov MV;Jones MR;Rouillard AD;Fernandez NF;Duan Q;Wang Z;Koplev S;Jenkins SL;Jagodnik KM;Lachmann A;McDermott MG;Monteiro CD;Gundersen GW;Ma'ayan A
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Ma'ayan A
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通讯作者：
Lee I
影响因子：
3.7
作者：
Eshoo, Mark W.;Crowder, Christopher C.;Aucott, John N.
通讯作者：
Aucott, John N.