Anti-cyclic citrullinated glucose-6-phosphate isomerase peptide-7 (CCG-7) antibodies were suppressed by biologics treatment and deposited to citrullinated proteins in CD68-positive cells in the RA synovium
Anti-cyclic citrullinated glucose-6-phosphate isomerase peptide-7 (CCG-7) antibodies were suppressed by biologics treatment and deposited to citrullinated proteins in CD68-positive cells in the RA synovium
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抗环瓜氨酸葡萄糖 6-磷酸异构酶肽 7 (CCG-7) 抗体被生物制剂治疗抑制，并沉积到 RA 滑膜 CD68 阳性细胞中的瓜氨酸蛋白上
DOI：
10.1080/14397595.2016.1270388
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发表时间：
2017
影响因子：
2.2
通讯作者：
Sumida Takayuki
中科院分区：
文献类型：
--
作者：
Umeda Naoto;Matsumoto Isao;Tanaka Yuki;Kawaguchi Hoshimi;Ebe Hiroshi;Kagami Yayoi;Ishigami Akihito;Sumida Takayuki
抗瓜氨酸肽抗体(ACPA)是类风湿性关节炎(RA)的特异性抗体，与疾病的严重程度相关。然而，抗环瓜氨酸多肽2(CCP-2)试验结果的变化与疾病活动性之间的关系尚未明确。[1-3]葡萄糖-6-磷酸异构酶抗体在小鼠中是致病的，[4]我们最近报道，抗瓜氨酸葡萄糖-6-磷酸异构酶多肽(CCG)-2和CCG-7抗体是RA的特异性生物标志物，使用肿瘤坏死因子拮抗剂可降低其疾病活动性；然而，抗瓜氨酸α-烯醇化酶(CEP)-1抗体并没有减少。[5]几种ACPA似乎是混合的和交叉反应的，但每种ACPA在不同生物处理下的波动还没有报道。[6]抗CCP2抗体已被证明沉积在培养的破骨细胞表面，[7]据报道，瓜氨酸在RA滑膜中表达。[8]然而，尚不清楚每种ACPA在滑膜中相互作用的位置。在这项研究中，我们调查了三种生物治疗(英夫利昔单抗、妥昔单抗和阿巴替普)对几种ACPA浓度的影响，并研究了它们在RA患者滑膜中的沉积情况。这些患者由筑波大学医院的风湿病学家根据2010年类风湿性关节炎分类标准进行诊断。[9]滑膜样本也采集自ACPA阳性的RA患者。[4]用三种环瓜氨酸多肽作为抗原：CCG-2、CCG-7和CEP-1。所有血浆样本均在术后6个月内采集。
Anti-citrullinated peptide antibodies (ACPA) are specific to rheumatoid arthritis (RA) and associated with disease severity. The correlation between changes in anti-cyclic citrullinated peptide 2 (CCP-2) test results and disease activity, however, has not been clearly defined.[1-3] Antibodies to glucose-6-phosphate isomerase are pathogenic in mice,[4] we recently reported that anti-citrullinated glucose-6-phosphate isomerase peptide (CCG)-2 and CCG-7 antibodies are specific biomarkers of RA and are decreased by using a TNF antagonist with an associated reduction in disease activity; however, anti-citrullinated alpha enolase peptide (CEP)-1 antibodies are not decreased.[5] Several ACPAs appear to be mixed and cross reactive, but fluctuation of each ACPA with different biologic treatments has not been reported.[6] Anti-CCP2 antibodies have been shown to be deposited on the surface of cultured osteoclasts,[7] and citrulline has been reported to be expressed in the RA synovium.[8] However, it is not clear where within the synovium each ACPA interacts. In this study, we investigated the fluctuation of several ACPAs by three kinds of biologic treatments (infliximab, tocilizumab and abatacept) and studied their deposition to the synovium in RA patients.After informed consent was obtained, plasma samples were obtained from 132 ACPA-positive RA patients. The patients had been diagnosed by rheumatologists at the University of Tsukuba Hospital according to 2010 Rheumatoid arthritis classification criteria.[9] Synovium samples were also collected from ACPA-positive RA patients. ACPAs were measured by ELISA using previously reported protocols.[4] Three cyclic citrullinated peptides were used as antigens: CCG-2, CCG-7 and CEP-1. All plasma samples were obtained within 6 months of the
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DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
D. Aletaha;T. Neogi;A. Silman;Julia Funovits;D. Felson;C. Bingham;N. Birnbaum;G. Burmester;V. Byker
通讯作者：
D. Aletaha;T. Neogi;A. Silman;Julia Funovits;D. Felson;C. Bingham;N. Birnbaum;G. Burmester;V. Byker
肽基精氨酸脱亚胺酶 4 (PADI4) 和瓜氨酸蛋白在类风湿性关节炎滑膜组织中的定位
DOI：
--
发表时间：
2006
期刊：
Rheumatology 44・1
影响因子：
--
作者：
Chang X;Yamada R;Suzuki A;Sawada T;Yoshino S;Tokuhiro S;Yamamoto K
通讯作者：
Yamamoto K
影响因子：
27.4
作者：
Ioan-Facsinay, Andreea;el-Bannoudi, Hanane;Toes, Rene E. M.
通讯作者：
Toes, Rene E. M.
影响因子：
3.4
作者：
Landmann, Thoren;Kehl, Gabriele;Bergner, Raoul
通讯作者：
Bergner, Raoul
影响因子：
27.4
作者：
Krishnamurthy A;Joshua V;Haj Hensvold A;Jin T;Sun M;Vivar N;Ytterberg AJ;Engström M;Fernandes-Cerqueira C;Amara K;Magnusson M;Wigerblad G;Kato J;Jiménez-Andrade JM;Tyson K;Rapecki S;Lundberg K;Catrina SB;Jakobsson PJ;Svensson C;Malmström V;Klareskog L;Wähämaa H;Catrina AI
通讯作者：
Catrina AI