Hemostatic gelatin sponge is a superior matrix to matrigel for establishment of LNCaP human prostate cancer in nude mice.
Hemostatic gelatin sponge is a superior matrix to matrigel for establishment of LNCaP human prostate cancer in nude mice.
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DOI：
10.1002/pros.22520
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发表时间：
2012-11
期刊：
影响因子：
2.8
通讯作者：
Dong, Zhongyun
中科院分区：
文献类型：
--
作者：
Cui, Lingling;Chen, Pingping;Tan, Zongqing;Li, Wenjie;Dong, Zhongyun
基质胶，溶解的基底膜制剂，通常用于支持动物模型中的肿瘤发展。然而，由肿瘤细胞和Matrigel的混合物形成的肿瘤可能会有显著变化。本研究的目的是比较与基质胶混合或在明胶海绵中的LNCaP人前列腺癌细胞的肿瘤发展和生长。将LNCaP细胞与Matrigel混合或吸收到明胶海绵VETSPON中，并接种到裸鼠皮下。测定肿瘤发生率和生长速率。通过免疫组化（IHC）、免疫印迹和RT-PCR评价肿瘤病变中的基因表达和细胞生长和存活。所有接种VETSPON中的LNCaP细胞的小鼠（12/12）产生肿瘤，相比之下，70%（19/27）的注射有Matrigel的细胞的小鼠产生肿瘤。VETSPON植入物的肿瘤体积变化也较小。在两种类型的植入物建立的肿瘤中，在基因表达、细胞生长、凋亡和微血管密度方面没有观察到显著差异。然而，在第1天和第4天收集的样品中，Matrigel植入物中的细胞比VETSPON植入物中的细胞更多地被染色为切割的半胱天冬酶3和PARP 1阳性。VEGF-A、HIF-1α和Bcl-2的表达在早期VETSPON植入物中升高。这些数据表明，VETSPON在植入早期促进肿瘤细胞存活，并表明明胶海绵在支持裸鼠中人前列腺癌的发展和进展方面上级Matrigel。该模型可用于使用LNCaP细胞的裸鼠临床前研究。
Matrigels, solubilized basement membrane preparations, are often used to support tumor development in animal models. However, tumors formed by a mixture of tumor cells and Matrigel may vary significantly. The purpose of this study was to compare tumor development and growth of LNCaP human prostate cancer cells mixed with Matrigel or in gelatin sponges. LNCaP cells were mixed with Matrigel or absorbed into VETSPON, a gelatin sponge, and inoculated into the subcutis of nude mice. Tumor incidence and growth rate were determined. Gene expression and cell growth and survival in tumor lesions were evaluated by immunohistochemistry (IHC), immunoblotting, and RT-PCR. All mice (12/12) inoculated with LNCaP cells in VETSPON produced tumors, compared to 70% (19/27) of mice injected with the cells with Matrigel. Tumor volume also varied less with VETSPON implants. No significant differences were observed in gene expression, cell growth, apoptosis, and microvessel density in tumors established from the two types of implants. However, in samples collected on days 1 and 4, more cells in Matrigel implants than those in VETSPON implants were stained positive for cleaved-caspase 3 and -PARP1. Expression of VEGF-A, HIF-1α, and Bcl-2 was elevated in the early VETSPON implants. These data indicate that VETSPON promotes tumor cell survival at the early stage of implantation and suggest that the gelatin sponge is superior to Matrigel in supporting development and progression of human prostate cancer in nude mice. This model should be useful for preclinical studies in nude mice using LNCaP cells.
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