Associations between variants of FADS genes and omega-3 and omega-6 milk fatty acids of Canadian Holstein cows.
Associations between variants of FADS genes and omega-3 and omega-6 milk fatty acids of Canadian Holstein cows.
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FADS基因的变体与加拿大荷斯坦奶牛的Omega-3和Omega-6牛奶脂肪酸之间的关联。
DOI：
10.1186/1471-2156-15-25
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发表时间：
2014-02-17
期刊：
影响因子：
2.9
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Ibeagha-Awemu EM;Akwanji KA;Beaudoin F;Zhao X
脂肪酸去饱和酶1(FADS1)和2(FADS2)基因分别编码Delta-5和Delta-6去饱和酶，它们是合成多不饱和omega-3和omega-6脂肪酸(FAs)的限速酶。Omega-3和-6脂肪酸以及共轭亚油酸(CLA)存在于牛乳中，对人类的健康有积极的影响。对人类的研究表明，FADS1和2基因的遗传变异与血浆和组织中omega-3和-6脂肪酸的浓度有显著关系。这项研究的目的是评估这两个基因在加拿大荷斯坦奶牛中的序列变异程度，以及序列变异与牛奶中促进健康的脂肪酸之间的关联。在FADS1外显子8的同义突变(FADS1-07，rs42187261,306Tyr >； Tyr)、FADS2外显子7的非同义突变(FADS2-14，rs211580559,294Ala >； val)、剪接位点SNP(FADS2-05，rs211263660)、3‘UTRSNP(FADS2-23，rs109772589)和3’UTRSNP(FADS2-19，rs2169303)。关联分析显示，七个被测试的SNPs中有三个与几个FAs有显著关系。FADS_2-23(Rs109772589)与两个omega-6脂肪酸(同质亚麻酸[C20：3n6]和花生四烯酸[C20：4n6])、fads_1-07(Rs42187261)和一个omega-3脂肪酸(二十碳五烯酸，C20：5n3)和三十烷酸(C23：0)，以及一个内含子SNP，FADS_1-01(Rs136261927)和C20：3n6之间存在显著的关联(FDR P <； 0.05)。我们的研究表明，FADS1和FADS2基因中的三个SNP(3‘非编码区内的一个SNP、一个同义SNP和一个内含子SNP)与加拿大荷斯坦奶牛乳中的三个多不饱和脂肪酸呈正相关，这表明同义和非编码区变异可能参与了FA的合成。这些SNPs可以作为潜在的遗传标记，用于提高牛奶中有益于人类健康的脂肪酸含量。
Fatty acid desaturase 1 (FADS1) and 2 (FADS2) genes code respectively for the enzymes delta-5 and delta-6 desaturases which are rate limiting enzymes in the synthesis of polyunsaturated omega-3 and omega-6 fatty acids (FAs). Omega-3 and-6 FAs as well as conjugated linoleic acid (CLA) are present in bovine milk and have demonstrated positive health effects in humans. Studies in humans have shown significant relationships between genetic variants in FADS1 and 2 genes with plasma and tissue concentrations of omega-3 and-6 FAs. The aim of this study was to evaluate the extent of sequence variations within these two genes in Canadian Holstein cows as well as the association between sequence variants and health promoting FAs in milk. Thirty three SNPs were detected within the studied regions of genes including a synonymous mutation (FADS1-07, rs42187261, 306Tyr > Tyr) in exon 8 of FADS1, a non-synonymous mutation (FADS2-14, rs211580559, 294Ala > Val) within FADS2 exon 7, a splice site SNP (FADS2-05, rs211263660), a 3′UTR SNP (FADS2-23, rs109772589), and another 3′UTR SNP with an effect on a microRNA binding site within FADS2 gene (FADS2-19, rs210169303). Association analyses showed significant relations between three out of seven tested SNPs and several FAs. Significant associations (FDR P < 0.05) were recorded between FADS2-23 (rs109772589) and two omega-6 FAs (dihomogamma linolenic acid [C20:3n6] and arachidonic acid [C20:4n6]), FADS1-07 (rs42187261) and one omega-3 FA (eicosapentaenoic acid, C20:5n3) and tricosanoic acid (C23:0), and one intronic SNP, FADS1-01 (rs136261927) and C20:3n6. Our study has demonstrated positive associations between three SNPs within FADS1 and FADS2 genes (a SNP within the 3’UTR, a synonymous SNP and an intronic SNP), with three milk PUFAs of Canadian Holstein cows thus suggesting possible involvement of synonymous and non-coding region variants in FA synthesis. These SNPs may serve as potential genetic markers in breeding programs to increase milk FAs that are of benefit to human health.
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影响因子：
30.8
作者：
通讯作者：
--
与人类血液低密度脂蛋白胆固醇、高密度脂蛋白胆固醇或甘油三酯相关的六个新位点。
影响因子：
30.8
作者：
Kathiresan, Sekar;Melander, Olle;Guiducci, Candace;Surti, Aarti;Burtt, Noel P.;Rieder, Mark J.;Cooper, Gregory M.;Roos, Charlotta;Voight, Benjamin F.;Havulinna, Aki S.;Wahlstrand, Bjorn;Hedner, Thomas;Corella, Dolores;Tai, E. Shyong;Ordovas, Jose M.;Berglund, Goran;Vartiainen, Erkki;Jousilahti, Pekka;Hedblad, Bo;Taskinen, Marja-Riitta;Newton-Cheh, Christopher;Salomaa, Veikko;Peltonen, Leena;Groop, Leif;Altshuler, David M.;Orho-Melander, Marju
通讯作者：
Orho-Melander, Marju
影响因子：
2
作者：
Macaya, D.;Katsanis, S. H.;Cutting, G. R.
通讯作者：
Cutting, G. R.
DOI：
10.1080/00288233.1990.10428440
发表时间：
1990-01-01
影响因子：
1.8
作者：
MONTGOMERY, GW;SISE, JA
通讯作者：
SISE, JA
影响因子：
2.4
作者：
Alim, M. A.;Wang, P.;Sun, D. X.
通讯作者：
Sun, D. X.