Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine.
Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine.
复制标题
DOI：
10.1093/nar/gkm657
复制
发表时间：
2007
影响因子：
14.9
通讯作者：
Piccirilli JA
中科院分区：
文献类型：
--
作者：
Dai Q;Fong R;Saikia M;Stephenson D;Yu YT;Pan T;Piccirilli JA
Over 100 chemical types of RNA modifications have been identified in thousands of sites in all three domains of life. Recent data suggest that modifications function synergistically to mediate biological function, and that cells may coordinately modulate modification levels for regulatory purposes. However, this area of RNA biology remains largely unexplored due to the lack of robust, high-throughput methods to quantify the extent of modification at specific sites. Recently, we developed a facile enzymatic ligation-based method for detection and quantitation of methylated 2′-hydroxyl groups within RNA. Here we exploit the principles of molecular recognition and nucleic acid chemistry to establish the experimental parameters for ligation-based detection and quantitation of pseudouridine (Ψ) and N6-methyladenosine (m6A), two abundant modifications in eukaryotic rRNA/tRNA and mRNA, respectively. Detection of pseudouridylation at several sites in the large subunit rRNA derived from yeast demonstrates the feasibility of the approach for analysis of pseudouridylation in biological RNA samples.
登录
查看更多内容
影响因子：
14.9
作者：
McCloskey JA;Rozenski J
通讯作者：
Rozenski J
影响因子：
14.9
作者：
Yarian, CS;Basti, MM;Agris, PF
通讯作者：
Agris, PF
影响因子：
1.8
作者：
Höbartner, C;Kreutz, C;Micura, R
通讯作者：
Micura, R
DOI：
10.1007/b106848
发表时间：
2005-01-01
期刊：
FINE-TUNING OF RNA FUNCTIONS BY MODIFICATION AND EDITING
影响因子：
--
作者：
Grosjean, H
通讯作者：
Grosjean, H
DOI：
10.1007/b106365
发表时间：
2005-01-01
期刊：
FINE-TUNING OF RNA FUNCTIONS BY MODIFICATION AND EDITING
影响因子：
--
作者：
Bokar, JA
通讯作者：
Bokar, JA