A qrr noncoding RNA deploys four different regulatory mechanisms to optimize quorum-sensing dynamics.
A qrr noncoding RNA deploys four different regulatory mechanisms to optimize quorum-sensing dynamics.
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DOI：
10.1016/j.cell.2014.11.051
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发表时间：
2015-01-15
期刊：
影响因子：
64.5
通讯作者：
Bassler BL
中科院分区：
文献类型：
--
作者：
Feng L;Rutherford ST;Papenfort K;Bagert JD;van Kessel JC;Tirrell DA;Wingreen NS;Bassler BL
群体感应是细菌用来在个体和社会生活方式之间转换的细胞间通信过程。在弧菌中，称为 Qrr sRNA 的同源小 RNA 在群体感应通路的中心发挥作用。 Qrr sRNA 调节多个 mRNA 靶标，包括编码群体感应调节成分 luxR、luxO、luxM 和 aphA 的靶标。我们发现，代表性的 Qrr，Qrr3，使用四种不同的机制来控制其特定靶点：Qrr3 sRNA 通过催化降解抑制 luxR，通过耦合降解抑制 luxM，通过隔离抑制 luxO，并在 sRNA 本身降解时通过暴露核糖体结合位点来激活 aphA。 Qrr3 与每个 mRNA 靶点形成不同的碱基配对相互作用，特定的配对策略决定了发生哪种调节机制。结合数学模型和实验表明，所采用的特定 Qrr 调节机制控制着目标 mRNA 调节的效力、动态和竞争，进而定义了整体群体感应反应。
Quorum sensing is a cell-cell communication process that bacteria use to transition between individual and social lifestyles. In vibrios, homologous small RNAs called the Qrr sRNAs function at the center of quorum-sensing pathways. The Qrr sRNAs regulate multiple mRNA targets including those encoding the quorum-sensing regulatory components luxR, luxO, luxM, and aphA. We show that a representative Qrr, Qrr3, uses four distinct mechanisms to control its particular targets: Qrr3 sRNA represses luxR through catalytic degradation, represses luxM through coupled degradation, represses luxO through sequestration, and activates aphA by revealing the ribosome-binding site while the sRNA itself is degraded. Qrr3 forms different base-pairing interactions with each mRNA target, and the particular pairing strategy determines which regulatory mechanism occurs. Combined mathematical modeling and experiments show that the specific Qrr regulatory mechanism employed governs the potency, dynamics and competition of target mRNA regulation, which in turn, defines the overall quorum-sensing response.
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影响因子：
11.4
作者：
Shao, Yi;Feng, Lihui;Rutherford, Steven T.;Papenfort, Kai;Bassler, Bonnie L.
通讯作者：
Bassler, Bonnie L.
影响因子：
3.6
作者：
Moon K;Gottesman S
通讯作者：
Gottesman S
影响因子：
3.6
作者：
Plumbridge, Jacqueline;Bossi, Lionello;Figueroa-Bossi, Nara
通讯作者：
Figueroa-Bossi, Nara
影响因子：
3.4
作者：
Teng, Shu-Wen;Wang, Yufang;Ong, N. P.
通讯作者：
Ong, N. P.
DOI：
10.1073/pnas.032066599
发表时间：
2002-04-02
影响因子：
11.1
作者：
Massé, E;Gottesman, S
通讯作者：
Gottesman, S