Functional determinants of the quorum-sensing non-coding RNAs and their roles in target regulation.
Functional determinants of the quorum-sensing non-coding RNAs and their roles in target regulation.
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DOI：
10.1038/emboj.2013.155
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发表时间：
2013-07-31
期刊：
影响因子：
11.4
通讯作者：
Bassler, Bonnie L.
中科院分区：
文献类型：
--
作者：
Shao, Yi;Feng, Lihui;Rutherford, Steven T.;Papenfort, Kai;Bassler, Bonnie L.
群体感应是一种化学通讯过程，细菌利用它来控制集体行为，包括生物发光、生物膜形成和毒力因子的产生。在哈维弧菌中，5个称为Qrr1-5的同源小rna （sRNAs）控制群体感应转换。在这里，我们确定了16个Qrr srna的新靶点。诱变表明，Qrr sRNAs之间的特定序列差异决定了它们的靶特异性。结合生化和遗传分析的建模表明，所有5种Qrr sRNAs都具有4个茎环：第一个茎环对于与靶标子集的碱基配对至关重要。这个茎环也保护Qrr sRNAs免受RNase e介导的降解。第二个茎环包含与大多数目标mrna进行碱基配对所需的保守序列。第三茎环在碱基配对和稳定性中起辅助作用。第四个茎环作为rho无关终止子。在群体感应调控中，Qrr srnas控制的基因对群体感应自诱导剂的反应最为迅速。Qrr srna在整个弧菌中都是保守的，因此这项工作的见解可以普遍应用于弧菌群体感应。
Quorum sensing is a chemical communication process that bacteria use to control collective behaviours including bioluminescence, biofilm formation, and virulence factor production. In Vibrio harveyi, five homologous small RNAs (sRNAs) called Qrr1–5, control quorum-sensing transitions. Here, we identify 16 new targets of the Qrr sRNAs. Mutagenesis reveals that particular sequence differences among the Qrr sRNAs determine their target specificities. Modelling coupled with biochemical and genetic analyses show that all five of the Qrr sRNAs possess four stem-loops: the first stem-loop is crucial for base pairing with a subset of targets. This stem-loop also protects the Qrr sRNAs from RNase E-mediated degradation. The second stem-loop contains conserved sequences required for base pairing with the majority of the target mRNAs. The third stem-loop plays an accessory role in base pairing and stability. The fourth stem-loop functions as a rho-independent terminator. In the quorum-sensing regulon, Qrr sRNAs-controlled genes are the most rapid to respond to quorum-sensing autoinducers. The Qrr sRNAs are conserved throughout vibrios, thus insights from this work could apply generally to Vibrio quorum sensing.
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