A combination of CMC and α-MSH inhibited ROS activated NLRP3 inflammasome in hyperosmolarity stressed HCECs and scopolamine-induced dry eye rats.
A combination of CMC and α-MSH inhibited ROS activated NLRP3 inflammasome in hyperosmolarity stressed HCECs and scopolamine-induced dry eye rats.
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CMC 和 α-MSH 组合抑制高渗透压应激 HCEC 和东莨菪碱诱导的干眼大鼠中 ROS 激活的 NLRP3 炎症小体
DOI：
10.1038/s41598-020-80849-2
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发表时间：
2021-01-13
影响因子：
4.6
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Lv Y;Chu C;Liu K;Ru Y;Zhang Y;Lu X;Gao Y;Zhang C;Zhao S
干眼症（DE）中涉及的一个重要机制是泪液高渗性与炎症严重程度之间的关联。DE中的炎症可能由NLRP 3炎性体介导，其通过暴露于活性氧（ROS）而被激活。羧甲基纤维素（CMC）和α-黑素细胞刺激素（α-MSH）联合应用可能通过这一机制影响DE，从而避免了信号药物的缺陷。在这项研究中，我们评估了CMC联合α-MSH治疗是否可以改善眼表功能;我们发现它促进泪液分泌，降低荧光素钠染色密度，增加结膜杯状细胞数量，减少角膜凋亡细胞数量。对潜在机制的研究表明，联合治疗的协同作用通过降低ROS水平和抑制人角膜上皮细胞中的NLRP 3炎性体来减轻DE炎症。这些结果表明，CMC + α-MSH联合治疗可通过降低ROS水平和抑制NLRP 3信号转导来改善DE患者的病变并恢复眼表功能。
An important mechanism involved in dry eye (DE) is the association between tear hyperosmolarity and inflammation severity. Inflammation in DE might be mediated by the NLRP3 inflammasome, which activated by exposure to reactive oxygen species (ROS). A combination of carboxymethylcellulose (CMC) and α-melanocyte stimulating hormone (α-MSH) may influence DE through this mechanism, thus avoiding defects of signal drug. In this study, we assessed whether treatment comprising CMC combined with α-MSH could ameliorate ocular surface function; we found that it promoted tear secretion, reduced the density of fluorescein sodium staining, enhanced the number of conjunctival goblet cells, and reduced the number of corneal apoptotic cells. Investigation of the underlying mechanism suggested that the synergistic effect of combined treatment alleviated DE inflammation through reduction of ROS level and inhibition of the NLRP3 inflammasome in human corneal epithelial cells. These findings indicate that combined CMC + α-MSH treatment could ameliorate lesions and restore ocular surface function in patients with DE through reduction of ROS level and inhibition of NLRP3 signalling.
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DOI：
10.1038/eye.2017.73
发表时间：
2017-10
期刊：
Eye (London, England)
影响因子：
--
作者：
Labetoulle M;Chiambaretta F;Shirlaw A;Leaback R;Baudouin C
通讯作者：
Baudouin C
影响因子：
3.5
作者：
Guzman-Aranguez, Ana;Perez de Lara, Maria J.;Pintor, Jesus
通讯作者：
Pintor, Jesus
影响因子：
5.6
作者：
Tsang, Julia;Jiang, Shuiping;Lechler, Robert I.
通讯作者：
Lechler, Robert I.
DOI：
10.3980/j.issn.2222-3959.2015.02.01
发表时间：
2015-04-18
影响因子：
1.4
作者：
Lee, Long Soo;Lee, Scans Uk;Lee, Ji-Eun
通讯作者：
Lee, Ji-Eun
影响因子：
3.3
作者：
Clemson CM;Yost J;Taylor AW
通讯作者：
Taylor AW