Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody

Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody
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使用 DNA 探针和早期核抗原单克隆抗体检测贝壳瓶培养物中的巨细胞病毒

DOI:
10.1128/jcm.26.9.1895-1897.1988
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发表时间:
1988
影响因子:
9.4
通讯作者:
T. Gansler
T. Gansler
中科院分区:
医学2区
文献类型:
--
作者:
A. A. Scott;K. A. Walker;L. M. Hennigar;C. Williams;J. Manos;T. Gansler

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评价了原位生物素化DNA探针试验作为抗巨细胞病毒早期核抗原间接免疫荧光和壳瓶培养物中巨细胞病毒感染单层细胞病变效应的辅助方法。病毒感染检测早期核抗原间接免疫荧光法在24小时和DNA探针测定和壳小瓶细胞病变效应在5天。
An in situ biotinylated DNA probe assay was evaluated as an adjunct to anti-cytomegalovirus early nuclear antigen indirect immunofluorescence and cytopathic effect on cytomegalovirus-infected monolayers in shell vial cultures. Viral infection was detected by early nuclear antigen indirect immunofluorescence at 24 h and by DNA probe assay and shell vial cytopathic effect at 5 days.
一种单步银增强方法,可通过原位杂交和免疫过氧化物酶检测快速诊断福尔马林固定、石蜡包埋的组织切片中的巨细胞病毒感染。
DOI: 10.1177/34.12.3023477
发表时间: 1986
期刊: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子: --
作者:
Przepiorka,D;Myerson,D
通讯作者: Myerson,D
DOI: 10.1093/infdis/150.2.272
发表时间: 1984
期刊: The Journal of infectious diseases
影响因子: --
作者:
Myerson,D;Hackman,RC;Meyers,JD
通讯作者: Meyers,JD