Identification of a myometrial molecular profile for dystocic labor.
Identification of a myometrial molecular profile for dystocic labor.
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DOI：
10.1186/1471-2393-11-74
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发表时间：
2011-10-16
影响因子：
3.1
通讯作者：
O'Herlihy C
中科院分区：
文献类型：
--
作者：
Brennan DJ;McGee SF;Rexhepaj E;O'Connor DP;Robson M;O'Herlihy C
在未产妇中，最常见的剖宫产指征是继发于子宫肌层收缩力无效的难产。本研究的目的是确定与难产相关的子宫肌层分子谱。子宫肌层活组织检查取自因难产而接受下段CS的初产妇女（n = 4）和在第二阶段接受CS的对照妇女（n = 4）的上切口边缘，这些妇女在第一产程期间表现出有效的子宫活动。所有患者均为自然分娩（非引产），并在分娩期间接受催产素以加速分娩。从活组织检查中提取RNA并与AffyphineHuGene U133 A Plus 2微阵列杂交。使用定量SYBR绿色实时PCR进行内部验证。70个基因在两组间差异表达。难产组有58个基因表达下调。基因本体分析显示，58个下调基因中有12个参与免疫应答。这些包括（ERAP 2，（8.67倍变化（FC））HLA-DQB 1（7.88 FC）CD 28（2.60 FC），LILRA 3（2.87 FC）和TGFBR 3（2.1 FC））。分层聚类表明来自难产和非难产的样品之间的总体基因表达模式的差异。RT-PCR检测ERAP 2、CD 28、LILRA 3和TGFBR 3 4个基因的表达。差异表达的基因表明难产的免疫反应的重要作用，并可能提供新的诊断方法和潜在的产时治疗靶点的重要指标。
The most common indication for cesarean section (CS) in nulliparous women is dystocia secondary to ineffective myometrial contractility. The aim of this study was to identify a molecular profile in myometrium associated with dystocic labor. Myometrial biopsies were obtained from the upper incisional margins of nulliparous women undergoing lower segment CS for dystocia (n = 4) and control women undergoing CS in the second stage who had demonstrated efficient uterine action during the first stage of labor (n = 4). All patients were in spontaneous (non-induced) labor and had received intrapartum oxytocin to accelerate labor. RNA was extracted from biopsies and hybridized to Affymetrix HuGene U133A Plus 2 microarrays. Internal validation was performed using quantitative SYBR Green Real-Time PCR. Seventy genes were differentially expressed between the two groups. 58 genes were down-regulated in the dystocia group. Gene ontology analysis revealed 12 of the 58 down-regulated genes were involved in the immune response. These included (ERAP2, (8.67 fold change (FC)) HLA-DQB1 (7.88 FC) CD28 (2.60 FC), LILRA3 (2.87 FC) and TGFBR3 (2.1 FC)) Hierarchical clustering demonstrated a difference in global gene expression patterns between the samples from dystocic and non-dystocic labours. RT-PCR validation was performed on 4 genes ERAP2, CD28, LILRA3 and TGFBR3 These findings suggest an underlying molecular basis for dystocia in nulliparous women in spontaneous labor. Differentially expressed genes suggest an important role for the immune response in dystocic labor and may provide important indicators for new diagnostic assays and potential intrapartum therapeutic targets.
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影响因子：
1.8
作者：
Kim, Lena Heesun;Cheng, Yvonne W.;Caughey, Aaron B.
通讯作者：
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影响因子：
6.1
作者：
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DOI：
10.1111/j.0001-6349.2005.00681.x
发表时间：
2005-07-01
影响因子：
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作者：
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
105.7
作者：
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通讯作者：
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