Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth.
Angiogenesis gene expression in murine endothelial cells during post-pneumonectomy lung growth.
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DOI：
10.1186/1465-9921-12-98
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发表时间：
2011-07-27
影响因子：
5.8
通讯作者：
Mentzer SJ
中科院分区：
文献类型：
--
作者：
Lin M;Chamoto K;Gibney BC;Lee GS;Collings-Simpson D;Houdek J;Konerding MA;Tsuda A;Mentzer SJ
虽然血管生长容易发生在全身支气管循环，血管生成在肺循环是罕见的。肺切除术后代偿性肺生长是一个假定肺泡毛细血管新生的实验模型。为了研究参与小鼠新肺泡形成的基因，我们研究了肺内皮细胞中血管生成基因的表达。左肺切除后，在术后第3、6、14和21天检查剩余的右肺，并与未手术和假开胸对照组进行比较。酶消化肺，并使用流式细胞术细胞分选分离CD 31+内皮细胞。使用定量实时聚合酶链反应（PCR）阵列评估CD31+内皮细胞的转录谱。聚焦于84个血管生成相关基因，我们鉴定了22个在肺切除术后21天内具有大于4倍调节和显著增强转录（p <0.05）的基因。22个基因的聚类分析表明，基因表达的变化并没有发生在一个单一的阶段，但在至少四个波的基因表达：一个波表明减少基因表达超过3天后，肺切除术和3个连续波的表达增加后第6天，第14天，和第21天肺切除术。这些发现表明，在代偿性肺生长过程中，基因相互作用的网络有助于血管生成。
Although blood vessel growth occurs readily in the systemic bronchial circulation, angiogenesis in the pulmonary circulation is rare. Compensatory lung growth after pneumonectomy is an experimental model with presumed alveolar capillary angiogenesis. To investigate the genes participating in murine neoalveolarization, we studied the expression of angiogenesis genes in lung endothelial cells. After left pneumonectomy, the remaining right lung was examined on days 3, 6, 14 and 21days after surgery and compared to both no surgery and sham thoracotomy controls. The lungs were enzymatically digested and CD31+ endothelial cells were isolated using flow cytometry cell sorting. The transcriptional profile of the CD31+ endothelial cells was assessed using quantitative real-time polymerase chain reaction (PCR) arrays. Focusing on 84 angiogenesis-associated genes, we identified 22 genes with greater than 4-fold regulation and significantly enhanced transcription (p <.05) within 21 days of pneumonectomy. Cluster analysis of the 22 genes indicated that changes in gene expression did not occur in a single phase, but in at least four waves of gene expression: a wave demonstrating decreased gene expression more than 3 days after pneumonectomy and 3 sequential waves of increased expression on days 6, 14, and 21 after pneumonectomy. These findings indicate that a network of gene interactions contributes to angiogenesis during compensatory lung growth.
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影响因子：
3.3
作者：
DEGRAFF, AC;GROVER, RF;MILLER, JM
通讯作者：
MILLER, JM
影响因子：
82.9
作者：
Compernolle, V;Brusselmans, K;Carmeliet, P
通讯作者：
Carmeliet, P
DOI：
10.1002/ar.21158
发表时间：
2010-07-01
影响因子：
2
作者：
Muehlfeld, Christian;Weibel, Ewald R.;Ochs, Matthias
通讯作者：
Ochs, Matthias
影响因子：
--
作者：
CADUFF, JH;FISCHER, LC;BURRI, PH
通讯作者：
BURRI, PH
影响因子：
1.7
作者：
Gibney BC;Lee GS;Houdek JP;Lin M;Miele LF;Chamoto K;Konerding MA;Tsuda A;Mentzer SJ
通讯作者：
Mentzer SJ