Influence of magnetic fields on magneto-aerotaxis.
Influence of magnetic fields on magneto-aerotaxis.
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DOI：
10.1371/journal.pone.0101150
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Faivre D
中科院分区：
文献类型：
--
作者：
Bennet M;McCarthy A;Fix D;Edwards MR;Repp F;Vach P;Dunlop JW;Sitti M;Buller GS;Klumpp S;Faivre D
细胞对物理化学微环境变化的反应对它们的生存至关重要。例如，细菌的趋磁性利用地球磁场和化学感应，帮助微生物向喜欢的栖息地移动。这种复杂的反应的研究缺乏一种方法，允许同时映射的化学环境和生物体的反应，并产生一个受控的生理磁场的能力。因此，我们开发了一个多模态显微镜平台，满足这些要求。利用同步荧光和高速成像结合扩散和趋气模型，我们的特点是磁趋气的gryphiswaldense。我们评估了磁场（方向;强度）对微自动定向带的形成和动态（大小、动态、位置）的影响。如先前描述的趋磁性模型，指向缺氧区的氧气梯度的磁场的应用导致增强的趋气性，甚至下降到地球的磁场强度。我们发现，无论是10倍的场强增加还是45°的倾斜都不会导致定向效率的显著变化。然而，当磁场强度为零或当磁场角度倾斜到90°时，磁趋空性效率急剧降低。磁趋气性的经典模型假设响应与氧梯度方向和磁场方向之间的角度差的余弦成比例。然而，我们的实验证据表明，这种行为比该模型中假设的更复杂，从而为研究开辟了新的途径。
The response of cells to changes in their physico-chemical micro-environment is essential to their survival. For example, bacterial magnetotaxis uses the Earth's magnetic field together with chemical sensing to help microorganisms move towards favoured habitats. The studies of such complex responses are lacking a method that permits the simultaneous mapping of the chemical environment and the response of the organisms, and the ability to generate a controlled physiological magnetic field. We have thus developed a multi-modal microscopy platform that fulfils these requirements. Using simultaneous fluorescence and high-speed imaging in conjunction with diffusion and aerotactic models, we characterized the magneto- aerotaxis of Magnetospirillum gryphiswaldense. We assessed the influence of the magnetic field (orientation; strength) on the formation and the dynamic of a micro-aerotactic band (size, dynamic, position). As previously described by models of magnetotaxis, the application of a magnetic field pointing towards the anoxic zone of an oxygen gradient results in an enhanced aerotaxis even down to Earth's magnetic field strength. We found that neither a ten-fold increase of the field strength nor a tilt of 45° resulted in a significant change of the aerotactic efficiency. However, when the field strength is zeroed or when the field angle is tilted to 90°, the magneto-aerotaxis efficiency is drastically reduced. The classical model of magneto-aerotaxis assumes a response proportional to the cosine of the angle difference between the directions of the oxygen gradient and that of the magnetic field. Our experimental evidence however shows that this behaviour is more complex than assumed in this model, thus opening up new avenues for research.
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影响因子：
10.8
作者：
Vach PJ;Brun N;Bennet M;Bertinetti L;Widdrat M;Baumgartner J;Klumpp S;Fratzl P;Faivre D
通讯作者：
Faivre D
影响因子：
64.8
作者：
Dreyfus, R;Baudry, J;Bibette, J
通讯作者：
Bibette, J
影响因子：
5
作者：
Heyen, U;Schüler, D
通讯作者：
Schüler, D
影响因子：
2.1
作者：
Oliphant, Travis E.
通讯作者：
Oliphant, Travis E.
影响因子：
64.8
作者：
Mitchell, Amir;Romano, Gal H.;Pilpel, Yitzhak
通讯作者：
Pilpel, Yitzhak