A longitudinal study of the diabetic skin and wound microbiome.
A longitudinal study of the diabetic skin and wound microbiome.
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作者：
Gardiner M;Vicaretti M;Sparks J;Bansal S;Bush S;Liu M;Darling A;Harry E;Burke CM
2型糖尿病是一种慢性健康疾病，与包括慢性足部溃疡和皮肤感染发病率增加在内的皮肤疾病相关。皮肤微生物组被认为在皮肤防御和免疫功能中发挥重要作用。糖尿病影响皮肤环境，这可能会扰乱皮肤微生物组，进而可能对皮肤感染和伤口愈合产生影响。本研究对2型糖尿病患者的皮肤和伤口微生物组进行了检测。
对8名患有慢性足部溃疡的2型糖尿病患者进行了为期10周的跟踪研究，每两周从足部皮肤（拭子）和伤口（拭子及清创组织）取样。同时对8名对照组受试者也进行了为期10周的跟踪研究，每两周从足部皮肤采集拭子。对样本进行DNA处理，并对16S rRNA基因进行PCR以及对V4区域进行测序。
糖尿病患者的皮肤微生物组多样性明显低于对照组皮肤。糖尿病患者和对照组皮肤的群落组成也存在显著差异，然而两组间最丰富的分类群是相似的，差异是由皮肤群落中丰度极低的成员所导致的。慢性伤口往往由皮肤中最丰富的葡萄球菌主导，而其他丰富的伤口分类群因患者而异。在伤口持续时间或愈合状况与任何特定分类群的丰度之间未发现显著相关性。
在这项与糖尿病相关的皮肤微生物组研究中观察到的主要差异是多样性显著降低。多样性降低的长期影响尚未完全了解，但通常与疾病状况有关。
Type II diabetes is a chronic health condition which is associated with skin conditions including chronic foot ulcers and an increased incidence of skin infections. The skin microbiome is thought to play important roles in skin defence and immune functioning. Diabetes affects the skin environment, and this may perturb skin microbiome with possible implications for skin infections and wound healing. This study examines the skin and wound microbiome in type II diabetes. Eight type II diabetic subjects with chronic foot ulcers were followed over a time course of 10 weeks, sampling from both foot skin (swabs) and wounds (swabs and debrided tissue) every two weeks. A control group of eight control subjects was also followed over 10 weeks, and skin swabs collected from the foot skin every two weeks. Samples were processed for DNA and subject to 16S rRNA gene PCR and sequencing of the V4 region. The diabetic skin microbiome was significantly less diverse than control skin. Community composition was also significantly different between diabetic and control skin, however the most abundant taxa were similar between groups, with differences driven by very low abundant members of the skin communities. Chronic wounds tended to be dominated by the most abundant skin Staphylococcus, while other abundant wound taxa differed by patient. No significant correlations were found between wound duration or healing status and the abundance of any particular taxa. The major difference observed in this study of the skin microbiome associated with diabetes was a significant reduction in diversity. The long-term effects of reduced diversity are not yet well understood, but are often associated with disease conditions.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Dowd SE;Wolcott RD;Sun Y;McKeehan T;Smith E;Rhoads D
通讯作者：
Rhoads D
影响因子：
15.5
作者：
Cope EK;Goldberg AN;Pletcher SD;Lynch SV
通讯作者：
Lynch SV
影响因子：
3.7
作者：
Cogen AL;Yamasaki K;Muto J;Sanchez KM;Crotty Alexander L;Tanios J;Lai Y;Kim JE;Nizet V;Gallo RL
通讯作者：
Gallo RL
DOI：
10.4161/derm.22028
发表时间：
2012-07-01
期刊：
Dermato-endocrinology
影响因子：
--
作者：
Gkogkolou P;Böhm M
通讯作者：
Böhm M