Unravelling the transcriptome profile of the Swine respiratory tract mycoplasmas.
Unravelling the transcriptome profile of the Swine respiratory tract mycoplasmas.
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DOI：
10.1371/journal.pone.0110327
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zaha A
中科院分区：
文献类型：
--
作者：
Siqueira FM;Gerber AL;Guedes RL;Almeida LG;Schrank IS;Vasconcelos AT;Zaha A
猪呼吸道纤毛上皮主要由猪支原体、絮状支原体和猪鼻支原体定植。而M. flocculare实际上是无症状的，M.和M.猪鼻感染可引起呼吸道疾病。关于转录本结构和基因丰度的信息提供了对基因功能和调控的有价值的见解，这在这些支原体种属中尚未在全基因组范围内进行分析。在这项研究中，我们报告了M。hypopalanum，M. flocculare和M. hyorhinis，其代表用于对转录库进行比较研究的数据。对于每个物种，产生三个cDNA文库，产生M的平均415，265，695，313和93，578个读数。hypopalanum，M. flocculare和M. hyorhinis，平均读长为274 bp。reads作图结果显示，预测基因的92%、98%和96%在M. hypopalanum，M. flocculare和M. hyorhinis基因组。此外，我们发现大多数基因是共表达的，证实了先前预测的转录单位。最后，我们的数据详细定义了RNA群体，在全基因组范围内绘制了转录本边界和转录单位结构。
The swine respiratory ciliary epithelium is mainly colonized by Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis. While colonization by M. flocculare is virtually asymptomatic, M. hyopneumoniae and M. hyorhinis infections may cause respiratory disease. Information regarding transcript structure and gene abundance provides valuable insight into gene function and regulation, which has not yet been analyzed on a genome-wide scale in these Mycoplasma species. In this study, we report the construction of transcriptome maps for M. hyopneumoniae, M. flocculare and M. hyorhinis, which represent data for conducting comparative studies on the transcriptional repertory. For each species, three cDNA libraries were generated, yielding averages of 415,265, 695,313 and 93,578 reads for M. hyopneumoniae, M. flocculare and M. hyorhinis, respectively, with an average read length of 274 bp. The reads mapping showed that 92%, 98% and 96% of the predicted genes were transcribed in the M. hyopneumoniae, M. flocculare and M. hyorhinis genomes, respectively. Moreover, we showed that the majority of the genes are co-expressed, confirming the previously predicted transcription units. Finally, our data defined the RNA populations in detail, with the map transcript boundaries and transcription unit structures on a genome-wide scale.
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枯草芽孢杆菌和相关物种中转录终止剂的预测。
影响因子：
4.3
作者：
de Hoon, Michiel J L;Makita, Yuko;Nakai, Kenta;Miyano, Satoru
通讯作者：
Miyano, Satoru
影响因子：
3.6
作者：
Burnett, TA;Dinkla, K;Djordjevic, SP
通讯作者：
Djordjevic, SP
影响因子：
3.2
作者：
Benders, GA;Powell, BC;Hutchison, CA
通讯作者：
Hutchison, CA
影响因子：
3.6
作者：
Deutscher, Ania T.;Jenkins, Cheryl;Djordjevic, Steven P.
通讯作者：
Djordjevic, Steven P.
影响因子：
56.9
作者：
Gueell, Marc;van Noort, Vera;Serrano, Luis
通讯作者：
Serrano, Luis