Ultrasound biomicroscopy for longitudinal studies of carotid plaque development in mice: validation with histological endpoints.
Ultrasound biomicroscopy for longitudinal studies of carotid plaque development in mice: validation with histological endpoints.
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DOI：
10.1371/journal.pone.0029944
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lennartz MR
中科院分区：
文献类型：
--
作者：
Harmon EY;Fronhofer V;Keller RS;Feustel PJ;Brosnan MJ;von der Thüsen JH;Loegering DJ;Lennartz MR
动脉粥样硬化每年造成数千名美国人死亡。斑块发展的颈动脉收缩模型最近被提出作为小鼠中不稳定斑块形成的模型。在这项研究中，我们1）验证超声生物显微镜（UBM）用于确定活体动物颈动脉斑块大小，狭窄百分比和斑块发展，2）确定UBM检测颈动脉收缩引起的血流变化的灵敏度和3）测试斑块形成是否可以从UBM测量的血流参数预测。在Apo E−/−小鼠中通过手术收缩诱导颈动脉斑块。每两周通过UBM对动脉进行成像，此时确定近端血流的PW-多普勒测量以及斑块长度和狭窄百分比。术后9周进行组织学检查。当与整个安装后尸检测量相比，UBM准确地报告了颈动脉斑块长度。基于横向B型UBM测量的狭窄百分比与组织学切片计算的狭窄百分比相关性良好。PW-Doppler显示收缩降低了最大收缩速度（vmax）和收缩速度峰值持续时间（ts/tt）。斑块前（术后2周）PW-Doppler参数（Vmax和ts/tt）与9周时的斑块长度相关，可预测斑块形成。初始PW-多普勒参数（vmax和ts/tt）与斑块长度的相关性确定了随后斑块发展所需的血流扰动程度，并证明了其预测斑块发展的能力。
Atherosclerosis is responsible for the death of thousands of Americans each year. The carotid constriction model of plaque development has recently been presented as a model for unstable plaque formation in mice. In this study we 1) validate ultrasound biomicroscopy (UBM) for the determination of carotid plaque size, percent stenosis, and plaque development in live animals, 2) determine the sensitivity of UBM in detecting changes in blood flow induced by carotid constriction and 3) test whether plaque formation can be predicted from blood flow parameters measured by UBM. Carotid plaques were induced by surgical constriction in Apo E−/− mice. Arteries were imaged bi-weekly by UBM, at which time PW-Doppler measurements of proximal blood flow, as well as plaque length and percent stenosis were determined. Histology was performed 9 weeks post surgery. When compared to whole mount post-mortem measurements, UBM accurately reported carotid plaque length. Percent stenosis, based on transverse B-mode UBM measurements, correlated well with that calculated from histological sections. PW-Doppler revealed that constriction reduced maximum systolic velocity (vmax) and duration of the systolic velocity peak (ts/tt). Pre-plaque (2 week post-surgery) PW-Doppler parameters (vmax and ts/tt) were correlated with plaque length at 9 weeks, and were predictive of plaque formation. Correlation of initiating PW-Doppler parameters (vmax and ts/tt) with resulting plaque length established the degree of flow disturbance required for subsequent plaque development and demonstrated its power for predicting plaque development.
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影响因子：
37.8
作者：
von der Thüsen, JH;van Berkel, TJC;Biessen, EAL
通讯作者：
Biessen, EAL
通过超声生物显微镜评估载脂蛋白 E 敲除小鼠的剪切应力与动脉粥样硬化病变之间的因果关系。
DOI：
10.1152/ajpheart.00308.2009
发表时间：
2010-06-01
影响因子：
4.8
作者：
Ding, Shi Fang;Ni, Mei;Zhang, Yun
通讯作者：
Zhang, Yun
影响因子：
20.3
作者：
Cheng, C;van Haperen, R;Krams, R
通讯作者：
Krams, R
DOI：
10.1161/atvbaha.109.196386
发表时间：
2010-01
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Kaufmann BA;Carr CL;Belcik JT;Xie A;Yue Q;Chadderdon S;Caplan ES;Khangura J;Bullens S;Bunting S;Lindner JR
通讯作者：
Lindner JR
影响因子：
5.3
作者：
Gan, Li-ming;Gronros, Julia;Fritsche-Danielson, Regina
通讯作者：
Fritsche-Danielson, Regina