Volumetric interferometric lattice light-sheet imaging.
Volumetric interferometric lattice light-sheet imaging.
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DOI：
10.1038/s41587-021-01042-y
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发表时间：
2021-11
影响因子：
46.9
通讯作者：
Pertsinidis A
中科院分区：
文献类型：
--
作者：
Cao B;Coelho S;Li J;Wang G;Pertsinidis A
Live-cell imaging with high spatiotemporal resolution and high detection sensitivity facilitates the study of the dynamics of cellular structure and function. However, extracting high-resolution 4D (3D space plus time) information from live cells remains challenging, because current methods are slow, require high peak excitation intensities or suffer from high out-of-focus background. Here we present 3D interferometric lattice light-sheet (3D-iLLS) imaging, a technique that requires low excitation light levels and provides high background suppression and substantially improved volumetric resolution by combining 4Pi interferometry with selective plane illumination. We demonstrate that 3D-iLLS has an axial resolution and single-particle localization precision of 100 nm (FWHM) and <10 nm (1σ), respectively. We illustrate the performance of 3D-iLLS in a range of systems: single messenger RNA molecules, nanoscale assemblies of transcription regulators in the nucleus, the microtubule cytoskeleton, and mitochondria organelles. The enhanced 4D resolution and increased signal-to-noise ratio (SNR) of 3D-iLLS will facilitate the analysis of biological processes at the sub-cellular level.
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DOI：
10.1126/science.aaq1392
发表时间：
2018-04-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Liu TL;Upadhyayula S;Milkie DE;Singh V;Wang K;Swinburne IA;Mosaliganti KR;Collins ZM;Hiscock TW;Shea J;Kohrman AQ;Medwig TN;Dambournet D;Forster R;Cunniff B;Ruan Y;Yashiro H;Scholpp S;Meyerowitz EM;Hockemeyer D;Drubin DG;Martin BL;Matus DQ;Koyama M;Megason SG;Kirchhausen T;Betzig E
通讯作者：
Betzig E
影响因子：
3.8
作者：
Reymond, Loic;Ziegler, Johannes;Wieser, Stefan
通讯作者：
Wieser, Stefan
高级成像 内吞和细胞骨架动力学的扩展分辨率结构照明成像
DOI：
10.1126/science.aab3500
发表时间：
2015-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li D;Shao L;Chen BC;Zhang X;Zhang M;Moses B;Milkie DE;Beach JR;Hammer JA 3rd;Pasham M;Kirchhausen T;Baird MA;Davidson MW;Xu P;Betzig E
通讯作者：
Betzig E
影响因子：
1.9
作者：
Baddeley, David;Carl, Christian;Cremer, Christoph
通讯作者：
Cremer, Christoph
影响因子：
64.5
作者：
Li, Jieru;Dong, Ankun;Pertsinidis, Alexandros
通讯作者：
Pertsinidis, Alexandros