Identifying circRNA-associated-ceRNA networks in the hippocampus of Aβ1-42-induced Alzheimer's disease-like rats using microarray analysis.
Identifying circRNA-associated-ceRNA networks in the hippocampus of Aβ1-42-induced Alzheimer's disease-like rats using microarray analysis.
复制标题
使用微阵列分析识别 Abeta1-42 诱导的阿尔茨海默病样大鼠海马中的 circRNA 相关 ceRNA 网络。
DOI：
10.18632/aging.101427
复制
发表时间：
2018-04-27
期刊：
影响因子：
--
通讯作者：
Peng W
中科院分区：
文献类型：
--
作者：
Wang Z;Xu P;Chen B;Zhang Z;Zhang C;Zhan Q;Huang S;Xia ZA;Peng W
阿尔茨海默病（AD）是世界范围内最常见的痴呆形式。越来越多的证据表明非编码RNA与AD相关的病理生理学密切相关。然而，这些ncRNA的作用在很大程度上仍然未知。在本研究中，我们使用微阵列分析技术来表征Aβ1-42诱导的AD模型大鼠海马组织中环状RNA（circRNA）、microRNA（miRNAs）和mRNA的表达模式，以整合相互作用数据，从而为AD的潜在机制提供新的见解。在AD大鼠的海马中，总共鉴定出555种circRNA、183种miRNA和319种mRNA显著失调（倍数变化≥ 2.0且p值< 0.05）。然后使用定量实时聚合酶链反应（qRT-PCR）来验证随机选择的circRNA、miRNA和mRNA的表达。接下来，进行GO和KEGG通路分析以进一步研究ncRNA的生物学功能和潜在机制。此外，我们构建了circRNA-miRNA和竞争性内源RNA（ceRNA）调控网络，以确定ncRNA和mRNA之间的功能相互作用。我们的研究结果表明，不同的ncRNA表达模式参与AD的发病机制。我们的发现为进一步研究AD发病机制提供了新的视角，并可能促进针对ncRNA的新型治疗方法的开发。
Alzheimer’s disease (AD) is the most common form of dementia worldwide. Accumulating evidence indicates that non-coding RNAs are strongly implicated in AD-associated pathophysiology. However, the role of these ncRNAs remains largely unknown. In the present study, we used microarray analysis technology to characterize the expression patterns of circular RNAs (circRNAs), microRNAs (miRNAs), and mRNAs in hippocampal tissue from Aβ1-42-induced AD model rats, to integrate interaction data and thus provide novel insights into the mechanisms underlying AD. A total of 555 circRNAs, 183 miRNAs and 319 mRNAs were identified to be significantly dysregulated (fold-change ≥ 2.0 and p-value < 0.05) in the hippocampus of AD rats. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to validate the expression of randomly-selected circRNAs, miRNAs and mRNAs. Next, GO and KEGG pathway analyses were performed to further investigate ncRNAs biological functions and potential mechanisms. In addition, we constructed circRNA-miRNA and competitive endogenous RNA (ceRNA) regulatory networks to determine functional interactions between ncRNAs and mRNAs. Our results suggest the involvement of different ncRNA expression patterns in the pathogenesis of AD. Our findings provide a novel perspective for further research into AD pathogenesis and might facilitate the development of novel therapeutics targeting ncRNAs.
登录
查看更多内容
DOI：
10.1002/wrna.1463
发表时间：
2018-03
期刊：
Wiley interdisciplinary reviews. RNA
影响因子：
--
作者：
Idda ML;Munk R;Abdelmohsen K;Gorospe M
通讯作者：
Gorospe M
竞争性内源性 RNA 网络与胃癌
影响因子：
4.3
作者：
Guo, Lei-Lei;Song, Chun-Hua;Wang, Kai-Juan
通讯作者：
Wang, Kai-Juan
影响因子：
3.2
作者：
Lane EM;Hohman TJ;Jefferson AL;Alzheimer’s Disease Neuroimaging Initiative
通讯作者：
Alzheimer’s Disease Neuroimaging Initiative
DOI：
10.1016/j.bbrc.2016.01.183
发表时间：
2016-02-26
影响因子：
3.1
作者：
Lin, Shao-Peng;Ye, Shan;Ma, Qiu-Jie
通讯作者：
Ma, Qiu-Jie
影响因子：
14
作者：
Gebhardt, Florian M.;Scott, Heather A.;Dodd, Peter R.
通讯作者：
Dodd, Peter R.