Structural variation and DNA methylation shape the centromere-proximal meiotic crossover landscape in Arabidopsis.
Structural variation and DNA methylation shape the centromere-proximal meiotic crossover landscape in Arabidopsis.
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DOI：
10.1186/s13059-024-03163-4
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发表时间：
2024-01-22
期刊：
影响因子：
12.3
通讯作者：
中科院分区：
文献类型：
--
作者：
着丝粒将着丝粒复合物装载到染色体上，介导纺锤体附着并在细胞分裂过程中实现分离。虽然着丝粒执行保守的细胞功能，但其潜在的DNA序列在物种内部和物种之间高度不同。尽管DNA序列存在差异，但在真核生物中，着丝粒也普遍受到减数分裂交叉重组的抑制。然而，抑制着丝粒交叉的遗传和表观遗传因素仍未完全确定。为了探索着丝粒-近端减数分裂重组景观，我们绘制了14,397个与完全组装的拟南芥（A. thaliana）基因组的交叉图谱。拟南芥着丝粒包括装载含有CENH3组蛋白变体的核小体的大碱基卫星重复序列。每条染色体包含一个约3-4兆碱基的结构多态性区域，该区域缺乏交叉，包括卫星阵列。这个多态区域的两侧是1-2兆基的低重组区。这些重组抑制区域富含Gypsy/Ty3反转录转座子，另外还含有具有高遗传多样性的表达基因，这些基因可以启动减数分裂重组，但不会交叉。我们以高分辨率绘制了CEN3附近的交叉图，它解决了点状着丝粒-近端热点，这些热点重叠了嵌入在异染色质中的基因岛。着丝粒被密集的DNA甲基化，重组景观在DNA甲基化突变体中被重塑。我们观察到，在CG （met1）和非CG （cmt3）突变体中，着丝粒低重组区分别减少和增加交叉，而核心非重组区仍然受到抑制。我们的工作将拟南拟南植物着丝粒的遗传和表观遗传组织以及侧翼的近着丝粒异染色质与围绕cenh3占据的卫星重复序列的交叉抑制区联系起来。在线版本包含补充材料，可在10.1186/s13059-024-03163-4获得。
Centromeres load kinetochore complexes onto chromosomes, which mediate spindle attachment and allow segregation during cell division. Although centromeres perform a conserved cellular function, their underlying DNA sequences are highly divergent within and between species. Despite variability in DNA sequence, centromeres are also universally suppressed for meiotic crossover recombination, across eukaryotes. However, the genetic and epigenetic factors responsible for suppression of centromeric crossovers remain to be completely defined. To explore the centromere-proximal meiotic recombination landscape, we map 14,397 crossovers against fully assembled Arabidopsis thaliana (A. thaliana) genomes. A. thaliana centromeres comprise megabase satellite repeat arrays that load nucleosomes containing the CENH3 histone variant. Each chromosome contains a structurally polymorphic region of ~3–4 megabases, which lack crossovers and include the satellite arrays. This polymorphic region is flanked by ~1–2 megabase low-recombination zones. These recombination-suppressed regions are enriched for Gypsy/Ty3 retrotransposons, and additionally contain expressed genes with high genetic diversity that initiate meiotic recombination, yet do not crossover. We map crossovers at high-resolution in proximity to CEN3, which resolves punctate centromere-proximal hotspots that overlap gene islands embedded in heterochromatin. Centromeres are densely DNA methylated and the recombination landscape is remodelled in DNA methylation mutants. We observe that the centromeric low-recombining zones decrease and increase crossovers in CG (met1) and non-CG (cmt3) mutants, respectively, whereas the core non-recombining zones remain suppressed. Our work relates the genetic and epigenetic organization of A. thaliana centromeres and flanking pericentromeric heterochromatin to the zones of crossover suppression that surround the CENH3-occupied satellite repeat arrays. The online version contains supplementary material available at 10.1186/s13059-024-03163-4.
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影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
影响因子：
4.5
作者：
Choi K;Reinhard C;Serra H;Ziolkowski PA;Underwood CJ;Zhao X;Hardcastle TJ;Yelina NE;Griffin C;Jackson M;Mézard C;McVean G;Copenhaver GP;Henderson IR
通讯作者：
Henderson IR
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.15252/embj.2020104858
发表时间：
2020-11-02
期刊：
The EMBO journal
影响因子：
--
作者：
Blackwell AR;Dluzewska J;Szymanska-Lejman M;Desjardins S;Tock AJ;Kbiri N;Lambing C;Lawrence EJ;Bieluszewski T;Rowan B;Higgins JD;Ziolkowski PA;Henderson IR
通讯作者：
Henderson IR
影响因子：
4.5
作者：
Girard C;Chelysheva L;Choinard S;Froger N;Macaisne N;Lemhemdi A;Mazel J;Crismani W;Mercier R
通讯作者：
Mercier R