MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin.
MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin.
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MiR-125b 通过靶向巢蛋白来协调神经干/祖细胞的细胞增殖、分化和迁移
DOI：
10.1186/1471-2202-13-116
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发表时间：
2012-09-28
期刊：
影响因子：
2.4
通讯作者：
Dai J
中科院分区：
文献类型：
--
作者：
Cui Y;Xiao Z;Han J;Sun J;Ding W;Zhao Y;Chen B;Li X;Dai J
背景
Background
The emerging concept is that microRNAs (miRNAs) play a central role in controlling stem cell self-renewal and fate determination by regulating the expression of stem cell regulators. miR-125b, one of neuronal miRNAs, recently was found to be necessary for neural differentiation of neural stem/progenitor cells (NS/PCs). However, the other specific biological role of miR-125b in NS/PCs is little known. We used rat NS/PCs as a model system to study the role of miR-125b in governing the behavior of NS/PCs.
Results
We report here the transfection of exogenous miR-125b inhibited proliferation of NS/PCs but promoted differentiation and migration. Whereas anti-miR-125b had the opposite effect. Similar results were observed when Nestin was knocked down by siRNA. Subsequently, we demonstrated that Nestin was a direct functional target of miR-125b. MiR-125b downregulates the expression of luciferase through Nestin 3’untranslated region (3’-UTR), and the regulation was abolished by mutations in the miR-125b binding site. MiR-125b targeted the 3'-UTR of Nestin and reduced the abundance of Nestin at both mRNA and protein levels.
Conclusion
The results provided new insight into the function by which miR-125b modulates NS/PCs proliferation, differentiation and migration. The data also indicated the regulatory role of miR-125b in NS/PCs might through the suppression of Nestin expression.
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影响因子：
4.8
作者：
Armstrong, Lyle
通讯作者：
Armstrong, Lyle
影响因子：
9.8
作者：
Jessberger S;Aigner S;Clemenson GD Jr;Toni N;Lie DC;Karalay O;Overall R;Kempermann G;Gage FH
通讯作者：
Gage FH
影响因子：
5.2
作者：
Kimura, Atsushi;Ohmori, Tsukasa;Sakata, Yoichi
通讯作者：
Sakata, Yoichi
影响因子：
4.3
作者：
Kawahara H;Imai T;Okano H
通讯作者：
Okano H
影响因子：
21.3
作者：
Rybak, Agnieszka;Fuchs, Heiko;Wulczyn, F. Gregory
通讯作者：
Wulczyn, F. Gregory