Molecular cloning and characterization of a trehalose-6-phosphate synthase/phosphatase from Dunaliella viridis
Molecular cloning and characterization of a trehalose-6-phosphate synthase/phosphatase from Dunaliella viridis
复制标题
绿色杜氏藻海藻糖 6-磷酸合酶/磷酸酶的分子克隆和表征
DOI：
10.1007/s11033-010-0354-1
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Rentao Song
中科院分区：
文献类型：
--
作者：
Nan Zhang;Fei Wang;Xiangzong Meng;Saifan Luo;Qiyun Li;Hongyun Dong;Zhengkai Xu;Rentao Song
杜氏藻是一类具有优异抗逆性的绿色藻类，是抗逆性研究的重要模式生物。本研究克隆了绿色杜氏藻（Dunaliella viridis）的一个TPS（海藻糖-6-磷酸合成酶）基因，命名为DvTPS（D. DvTPS cDNA全长2793 bp，编码930个氨基酸。DvTPS具有TPS和TPP结构域，属于Ⅱ类TPS/TPP融合基因家族。Southern印迹显示它在基因组中只有一个拷贝。基因组序列分析表明，该基因有18个外显子和17个内含子。DvTPS在不同的NaCl培养条件下都有组成性的高表达，但盐激可以诱导其表达。启动子分析表明，该基因启动子区含有10个应激反应元件（stress response element，STRE），这可能解释了该基因在盐胁迫下的诱导表达模式。酵母功能互补分析表明DvTPS既无TPS活性也无TPP活性。而DvTPS能提高酵母盐敏感突变株G19的耐盐性。我们的研究结果表明，尽管DvTPS与TPS/TPP具有显著的相似性，但其真实的生物学功能仍有待于揭示。
Dunaliella is a group of green algae with exceptional stress tolerance capability, and is considered as an important model organism for stress tolerance study. Here we cloned a TPS (trehalose-6-phosphate synthase) gene from Dunaliella viridis and designated it as DvTPS (D. viridis trehalose-6-phosphate synthase/phosphatase).The DvTPS cDNA contained an ORF of 2793 bp encoding 930 aa. DvTPS had both TPS and TPP domain and belonged to the Group II TPS/TPP fusion gene family. Southern blots showed it has a single copy in the genome. Genome sequence analysis revealed that it has 18 exons and 17 introns. DvTPS had a constitutive high expression level under various NaCl culture conditions, however, could be induced by salt shock. Promoter analysis indicated there were ten STREs (stress response element) in its promoter region, giving a possible explanation of its inducible expression pattern upon salt shock. Yeast functional complementation analysis showed that DvTPS had neither TPS nor TPP activity. However, DvTPS could improve the salt tolerance of yeast salt sensitive mutant G19. Our results indicated that despite DvTPS showed significant similarity with TPS/TPP, its real biological function is still remained to be revealed.
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影响因子：
11.6
作者：
Quintero, FJ;Garciadeblas, B;RodriguezNavarro, A
通讯作者：
RodriguezNavarro, A
影响因子：
7.4
作者：
Rodolfo Zentella;J. MASCORRO-GALLARDO;P. Dijck;J. Folch-Mallol;B. Bonini;C. V. Vaeck;Roberto A. Gaxiola-Roberto
通讯作者：
Rodolfo Zentella;J. MASCORRO-GALLARDO;P. Dijck;J. Folch-Mallol;B. Bonini;C. V. Vaeck;Roberto A. Gaxiola-Roberto
DOI：
10.1111/j.1432-1033.1994.tb19929.x
发表时间：
1994-01-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
HOTTIGER, T;DEVIRGILIO, C;WIEMKEN, A
通讯作者：
WIEMKEN, A
影响因子：
3.4
作者：
Avonce N;Mendoza-Vargas A;Morett E;Iturriaga G
通讯作者：
Iturriaga G
DOI：
--
发表时间：
2006
期刊：
Journal of Shanghai University
影响因子：
--
作者：
Wang Wei;Song Rentao
通讯作者：
Wang Wei;Song Rentao