Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis.
Metabolomics Study of Resina Draconis on Myocardial Ischemia Rats Using Ultraperformance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry Combined with Pattern Recognition Methods and Metabolic Pathway Analysis.
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超高效液相色谱/四极杆飞行时间质谱结合模式识别方法和代谢途径分析血龙树脂对心肌缺血大鼠的代谢组学研究
DOI：
10.1155/2013/438680
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Chai Y
中科院分区：
文献类型：
--
作者：
Qi Y;Gu H;Song Y;Dong X;Liu A;Lou Z;Fan G;Chai Y
龙血竭（从剑叶龙血树中分离出的鲜红色树脂，RD）已临床用于治疗心肌缺血（MI）多年。然而，其对心肌缺血的药理作用机制仍知之甚少。本研究旨在表征心肌缺血的血浆代谢谱，并利用基于超高效液相色谱/四极杆飞行时间质谱的代谢组学，结合模式识别方法和代谢途径分析，研究龙血竭对心肌缺血的作用机制。揭示了20种表征心肌缺血代谢谱的代谢物标志物，它们主要涉及氨酰-tRNA生物合成、苯丙氨酸、酪氨酸和色氨酸生物合成、血管平滑肌收缩、鞘脂代谢等。然而，经过龙血竭治疗后，7种心肌缺血代谢物标志物的水平发生了变化，包括植物鞘氨醇、二氢鞘氨醇、乙酰肉碱、环鸟苷酸、环腺苷酸、L - 酪氨酸和L - 缬氨酸，这表明龙血竭可能通过调节紊乱的血管平滑肌收缩、鞘脂代谢、苯丙氨酸代谢和支链氨基酸代谢来缓解心肌缺血。据我们所知，这是从代谢组学角度研究龙血竭治疗心肌缺血机制的首次综合研究。我们的研究结果对于更好地理解心肌缺血代谢谱非常有价值，并为探索龙血竭对心肌缺血的作用机制提供了新的见解。
Resina draconis (bright red resin isolated from Dracaena cochinchinensis, RD) has been clinically used for treatment of myocardial ischemia (MI) for many years. However, the mechanisms of its pharmacological action on MI are still poorly understood. This study aimed to characterize the plasma metabolic profiles of MI and investigate the mechanisms of RD on MI using ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry-based metabolomics combined with pattern recognition methods and metabolic pathway analysis. Twenty metabolite markers characterizing metabolic profile of MI were revealed, which were mainly involved in aminoacyl-tRNA biosynthesis, phenylalanine, tyrosine, and tryptophan biosynthesis, vascular smooth muscle contraction, sphingolipid metabolism, and so forth. After RD treatment, however, levels of seven MI metabolite markers, including phytosphingosine, sphinganine, acetylcarnitine, cGMP, cAMP, L-tyrosine, and L-valine, were turned over, indicating that RD is likely to alleviate MI through regulating the disturbed vascular smooth muscle contraction, sphingolipid metabolism, phenylalanine metabolism, and BCAA metabolism. To our best knowledge, this is the first comprehensive study to investigate the mechanisms of RD for treating MI, from a metabolomics point of view. Our findings are very valuable to gain a better understanding of MI metabolic profiles and provide novel insights for exploring the mechanisms of RD on MI.
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影响因子：
6.5
作者：
MADISON, KC;SWARTZENDRUBER, DC;DOWNING, DT
通讯作者：
DOWNING, DT
影响因子：
1.8
作者：
Nicholson, JK;Lindon, JC;Holmes, E
通讯作者：
Holmes, E
影响因子：
5.1
作者：
Gowda GA;Zhang S;Gu H;Asiago V;Shanaiah N;Raftery D
通讯作者：
Raftery D
DOI：
10.1016/j.bbagen.2007.08.014
发表时间：
2008-03-01
影响因子：
3
作者：
Gundewar, Susheel;Lefer, David J.
通讯作者：
Lefer, David J.
影响因子：
--
作者：
Jiang, Peng;Dai, Weixing;Zhang, Weidong
通讯作者：
Zhang, Weidong