Fucoidan stimulates cell division in the amebocyte-producing organ of the schistosome-transmitting snail Biomphalaria glabrata.
Fucoidan stimulates cell division in the amebocyte-producing organ of the schistosome-transmitting snail Biomphalaria glabrata.
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DOI：
10.1016/j.jip.2014.09.005
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发表时间：
2014-11
影响因子：
3.4
通讯作者：
Ransone, Kathryn A.
中科院分区：
文献类型：
--
作者：
Sullivan, John T.;Belloir, Joseph A.;Beltran, Roxxana V.;Grivakis, Aris;Ransone, Kathryn A.
将5 μl的10 mg/ml硫酸多糖λ卡拉胶、硫酸葡聚糖、岩藻依聚糖和肝素、非硫酸多糖海带多糖以及单糖L-岩藻糖和L-半乳糖的溶液注射到成年萨尔瓦多（抗阿米巴体）品系光滑双脐螺中，并在注射后24 h在组织切片中测量阿米巴细胞产生器官（APO）中的有丝分裂活性。在测试的物质中，只有岩藻依聚糖诱导有丝分裂活性升高。去硫酸化的岩藻依聚糖不是促有丝分裂的，表明硫酸基团是活性所必需的。血吸虫易感的M-线蜗牛具有最小的或没有造血组织在他们的载脂蛋白O，没有响应岩藻多糖。将幼萨尔瓦多螺浸泡在1或10 mg/ml的岩藻多糖溶液中3 h，浸泡后24 h的有丝分裂活性没有增加，这表明B的外部和消化道上皮细胞。光滑植物对该分子是不可渗透的。这些结果为曼氏血吸虫孢子囊的皮层和排泄分泌产物中的岩藻糖基化聚糖部分负责B的APO中有丝分裂活性增加的假设提供了支持。感染了这种吸虫或注射了它的提取物的glabrata。
Adult Salvador (schistosome-resistant) strain Biomphalaria glabrata snails were injected with 5 μl of 10 mg/ml solutions of the sulfated polysaccharides λ carageenan, dextran sulfate, fucoidan, and heparin, the nonsulfated polysaccharide laminarin, and the monosaccharides L-fucose and L-galactose, and mitotic activity in the amebocyte-producing organ (APO) was measured in histological sections at 24h post injection. Among the substances tested, only fucoidan induced elevated mitotic activity. Desulfated fucoidan was not mitogenic, indicating that sulfate groups are required for activity. Schistosome-susceptible M-line snails possessed minimal or no hematopoietic tissue in their APO, which did not respond to fucoidan. Immersion of juvenile Salvador snails in 1 or 10 mg/ml solutions of fucoidan for 3h did not elevate mitotic activity at 24h post immersion, suggesting that the external and digestive tract epithelia of B. glabrata are impermeable to this molecule. These results provide support for the hypothesis that fucosylated glycans on the tegument and in excretory-secretory products of sporocysts of Schistosoma mansoni are in part responsible for increased mitotic activity in the APO of B. glabrata infected with this trematode or injected with its extracts.
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影响因子：
1.3
作者：
Castillo, Maria G.;Wu, Xiao-Jun;Yoshino, Timothy P.
通讯作者：
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影响因子：
2.9
作者：
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DOI：
10.3390/molecules13081671
发表时间：
2008-08-12
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li B;Lu F;Wei X;Zhao R
通讯作者：
Zhao R
DOI：
10.1073/pnas.94.16.8691
发表时间：
1997-08-05
影响因子：
11.1
作者：
Adema, CM;Hertel, LA;Loker, ES
通讯作者：
Loker, ES