Genetic Diversity in Hawaiian Stream Macroinvertebrates
Genetic Diversity in Hawaiian Stream Macroinvertebrates
复制标题
夏威夷溪流大型无脊椎动物的遗传多样性
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
David W. F Oltz
中科院分区：
文献类型：
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作者：
Melanie H. Bebler;David W. F Oltz
夏威夷群岛是世界上地理上最孤立的岛链，有很高比例的特有植物和动物物种。我们研究了夏威夷溪流大型无脊椎动物的所有四种本地物种的DNA序列变异。这些种类都是淡水无游性发育，其中卵在淡水中产卵和孵化，所产生的幼虫分散在海洋沃茨中，幼虫后的幼鱼返回淡水溪流并生长至性成熟。本文所研究的4种虾类为2种米虾（Atyoida bisulcata Randall和Macrobrachium grandimanusRandall）和2种腹足类软体动物（Neritina granosa Sowerby和Neritina vespertina Sowerby）。从位于高岛最末端的两个岛屿（夏威夷岛和考艾岛）采集的每个物种的7 - 28个个体的线粒体细胞- Chrome氧化酶亚基I（COI）基因的约350 bp的序列。四个物种间核苷酸位点多样性的估计（范围，0.30% - 0.58%）和F ST的估计值（范围，-0.028至0.172）与以前的夏威夷淡水两栖鱼类和大型无脊椎动物物种的研究相当，也与非两栖虾的下目Caridea的核苷酸位点多样性相当（平均值为0.62%，N=40种）。最常见的序列单倍型在每个物种之间共享的两个岛屿。这些结果表明，在这些amendromous物种的人口规模是大到足以维持遗传多样性，幼虫扩散是足以防止岛屿特定的分支的发展。
The Hawaiian archipelago is the world's most geographi- cally isolated island chain, with a high proportion of endemic plant and animal species. We studied DNA sequence variation in all four native species of Hawaiian stream macroinvertebrates. These species all exhib- it freshwater amphidromous development, in which eggs are spawned and hatched in freshwater, the resulting larvae disperse in marine waters, and post-larval juveniles return to freshwater streams and grow to sexu- al maturity. The four species studied here were two caridean shrimps (Atyoida bisulcata Randall and Macrobrachium grandimanusRandall) and two gastropod molluscs (Neritina granosa Sowerby and Neritina vespertina Sowerby). Approximately 350 bp of the mitochondrial cyto- chrome oxidase subunit I (COI) gene was sequenced for 7 - 28 individ- uals of each species sampled from two islands (Hawai'i and Kaua'i) located at the extreme ends of the high islands. Estimates of nucleotide site diversity among the four species (range, 0.30% - 0.58%) and esti- mates of F ST (range, -0.028 to 0.172) were comparable to previous stud- ies of Hawaiian freshwater amphidromous fish and macroinvertebrate species, and also comparable to nucleotide site diversity among non- amphidromous shrimp of the infraorder Caridea (mean, 0.62%, N=40 species). The most common sequence haplotype in each species was shared between the two islands. These results suggest that population sizes in these amphidromous species are large enough to maintain genetic diversity, and that larval dispersal is sufficient to prevent the development of island-specific clades.
影响因子：
3.3
作者：
K. Crandall;Alan R. Templeton
通讯作者：
K. Crandall;Alan R. Templeton