Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated Wnt/β-catenin signal pathway.
Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated Wnt/β-catenin signal pathway.
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DOI：
10.1371/journal.pone.0078700
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Ji Q;Liu X;Fu X;Zhang L;Sui H;Zhou L;Sun J;Cai J;Qin J;Ren J;Li Q
白藜芦醇是从中草药虎杖中提取的一种有效的抗肿瘤药物，具有抑制结直肠癌侵袭和转移的作用，其中长链非编码转移相关肺腺癌转录本1（RNA-MALAT 1）也发挥着重要作用。利用MALAT 1慢病毒shRNA和过表达构建体在CRC衍生细胞系LoVo和HCT 116中的表达，我们证明了白藜芦醇对CRC的抗肿瘤作用是通过抑制Wnt/β-catenin信号传导，从而抑制其靶基因如c-Myc、MMP-7的表达以及MALAT 1的表达。具体地，白藜芦醇下调MALAT 1，导致β-catenin的核定位减少，从而减弱Wnt/β-catenin信号传导，这导致CRC侵袭和转移的抑制。我们的这一发现无疑为白藜芦醇在未来预防和治疗结直肠癌中的应用提供了重要的临床前证据。
Resveratrol, extracted from Chinese herbal medicine Polygonum cuspidatum, is known to inhibit invasion and metastasis of human colorectal cancer (CRC), in which long non-coding Metastasis Associated Lung Adenocarcinoma Transcript 1 (RNA-MALAT1) also plays an important role. Using MALAT1 lentiviral shRNA and over-expression constructs in CRC derived cell lines, LoVo and HCT116, we demonstrated that the anti-tumor effects of resveratrol on CRC are through inhibiting Wnt/β-catenin signaling, thus the expression of its target genes such as c-Myc, MMP-7, as well as the expression of MALAT1. In detail, resveratrol down-regulates MALAT1, resulting in decreased nuclear localization of β-catenin thus attenuated Wnt/β-catenin signaling, which leads to the inhibition of CRC invasion and metastasis. This finding of ours surely provides important pre-clinical evidence supporting future use of resveratrol in prevention and treatment of CRC.
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影响因子：
8
作者：
Lin, R.;Maeda, S.;Edgington, T. S.
通讯作者：
Edgington, T. S.
影响因子：
5.2
作者：
Iwai, Soichi;Yonekawa, Atsuko;Yura, Yoshiaki
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Yura, Yoshiaki
影响因子：
4.3
作者：
Riles, William L.;Erickson, Jason;Holian, Oksana
通讯作者：
Holian, Oksana
影响因子：
3.4
作者：
Lai, Ming-chun;Yang, Zhe;Zheng, Shu-sen
通讯作者：
Zheng, Shu-sen
影响因子：
3.3
作者：
Back JH;Zhu Y;Calabro A;Queenan C;Kim AS;Arbesman J;Kim AL
通讯作者：
Kim AL