Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conidia.
Transcriptomic analysis of the exit from dormancy of Aspergillus fumigatus conidia.
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曲霉曲霉分生孢子的休眠状态的出口的转录组分析。
DOI：
10.1186/1471-2164-9-417
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发表时间：
2008-09-16
期刊：
影响因子：
4.4
通讯作者：
Latge, Jean-Paul
中科院分区：
文献类型：
--
作者：
Lamarre, Claude;Sokol, Serguei;Debeaupuis, Jean-Paul;Henry, Christine;Lacroix, Celine;Glaser, Philippe;Coppee, Jean-Yves;Francois, Jean-Marie;Latge, Jean-Paul
曲霉病的建立依赖于肺内烟曲霉分生孢子的休眠和萌发。为了获得对分生孢子萌发早期步骤的分子机制的理解，我们使用由来自> 3，000个基因的PCR片段构建的宏阵列进行了转录组学分析（约为注释的A.烟曲霉基因组）。主要结果如下：（1）分生孢子中储存着预先包装好的mRNA转录本（27%的基因在休眠分生孢子中具有转录物;（ii）在37°C下在营养培养基中孵育诱导基因的上调和下调：在阵列上沉积的基因总数的19%被上调，而在休眠分生孢子中具有预包装mRNA的基因的22%被下调。规范;（iii）在萌发的前30分钟期间观察到大多数修饰，而在随后的小时期间发生非常少的基因表达修饰;（iv）一岁的分生孢子和一周龄的分生孢子在转录水平上表现相似。转录组学数据表明，退出休眠与从发酵代谢到呼吸代谢的转变以及向立即蛋白质合成的趋势有关。
Establishment of aspergillosis is depending upon the exit from dormancy and germination of the conidia of Aspergillus fumigatus in the lung. To gain an understanding of the molecular mechanisms underlying the early steps of conidial germination, we undertook a transcriptomic analysis using macroarrays constructed with PCR fragments from > 3,000 genes (around one third of the annotated A. fumigatus genome). Major results of this analysis are the following: (i) conidia stored pre-packaged mRNAs transcripts (27% of genes have transcripts in the resting conidia; (ii) incubation at 37°C in a nutritive medium induced up- and down-regulation of genes: 19% of the total number of genes deposited on the array were up-regulated whereas 22% of the genes with pre-packaged mRNA in the resting conidia were down-regulated; (iii) most modifications were seen during the first 30 min of germination whereas very little modification of gene expression occurred during the following hour; (iv) one-year old conidia and one-week old conidia behaved similarly at transcriptional level. Transcriptomic data indicate that the exit from dormancy is associated with a shift from a fermentative metabolism to a respiratory metabolism as well as a trend toward immediate protein synthesis.
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影响因子：
4.8
作者：
Brengues, M;Pintard, L;Lapeyre, B
通讯作者：
Lapeyre, B
影响因子：
2.9
作者：
Ghanem, M;Gadda, G
通讯作者：
Gadda, G
影响因子：
3.6
作者：
d'Enfert, C;Bonini, BM;Terenzi, HF
通讯作者：
Terenzi, HF
影响因子：
3.4
作者：
Avonce N;Mendoza-Vargas A;Morett E;Iturriaga G
通讯作者：
Iturriaga G
粗糙脉孢菌中的长寡聚体微阵列分析揭示了分生孢子萌发的生化和生理事件背后的转录程序。
影响因子：
14.9
作者：
Kasuga, T;Townsend, JP;Tian, CG;Gilbert, LB;Mannhaupt, G;Taylor, JW;Glass, NL
通讯作者：
Glass, NL