Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase.
Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase.
复制标题
平滑肌 Nox4 型 NADPH 氧化酶的促动脉粥样硬化作用
DOI：
10.1016/j.yjmcc.2016.01.020
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发表时间：
2016-03
影响因子：
5
通讯作者：
Zeng, Chunyu
中科院分区：
文献类型：
--
作者：
Tong, Xiaoyong;Khandelwal, Alok R.;Wu, Xiaojuan;Xu, Zaicheng;Yu, Weimin;Chen, Caiyu;Zhao, Wanzhou;Yang, Jian;Qin, Zhexue;Weisbrod, Robert M.;Seta, Francesca;Ago, Tetsuro;Lee, Kin Sing Stephen;Hammock, Bruce D.;Sadoshima, Junichi;Cohen, Richard A.;Zeng, Chunyu
Nox4-based NADPH oxidase is a major reactive oxygen species-generating enzyme in the vasculature, but its role in atherosclerosis remains controversial. Our goal was to investigate the role of smooth muscle Nox4 in atherosclerosis. Atherosclerosis-prone conditions (disturbed blood flow and Western diet) increased Nox4 mRNA level in smooth muscle of arteries. To address whether upregulated smooth muscle Nox4 under atherosclerosis-prone conditions was directly involved in the development of atherosclerosis, mice carrying a human Nox4 P437H dominant negative mutation (Nox4DN), specifically in smooth muscle, were generated on a FVB/N ApoE deficient genetic background to counter the effect of increased smooth muscle Nox4. Nox4DN significantly decreased aortic stiffness and atherosclerotic lesions, with no effect on blood pressure. Gene analysis indicated that soluble epoxide hydrolase 2 (sEH) was significantly downregulated in Nox4DN smooth muscle cells (SMC), at both mRNA and protein levels. Downregulation of sEH by siRNA decreased SMC proliferation and migration, and suppressed inflammation and macrophage adhesion to SMC. Downregulation of smooth muscle Nox4 inhibits atherosclerosis by suppressing sEH, which, at least in part, accounts for inhibition of SMC proliferation, migration and inflammation.
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影响因子：
5
作者：
Deng Y;Theken KN;Lee CR
通讯作者：
Lee CR
DOI：
10.1073/pnas.1002178107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Kuroda, Junya;Ago, Tetsuro;Sadoshima, Junichi
通讯作者：
Sadoshima, Junichi
影响因子：
7.4
作者：
Craige SM;Kant S;Reif M;Chen K;Pei Y;Angoff R;Sugamura K;Fitzgibbons T;Keaney JF Jr
通讯作者：
Keaney JF Jr
影响因子：
33.6
作者：
Chiu JJ;Chien S
通讯作者：
Chien S
DOI：
10.1083/jcb.200709049
发表时间：
2008-06-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen K;Kirber MT;Xiao H;Yang Y;Keaney JF Jr
通讯作者：
Keaney JF Jr