Expression of H-ras correlates with metastatic potential: evidence for direct regulation of the metastatic phenotype in 10T1/2 and NIH 3T3 cells
Expression of H-ras correlates with metastatic potential: evidence for direct regulation of the metastatic phenotype in 10T1/2 and NIH 3T3 cells
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H-ras 的表达与转移潜能相关：直接调节 10T1/2 和 NIH 3T3 细胞转移表型的证据
DOI：
10.1128/mcb.7.2.830-837.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
A. Greenberg
中科院分区：
文献类型：
--
作者：
S. Egan;G. McClarty;L. Jarolim;J. Wright;I. Spiro;G. Hager;A. Greenberg
使用三种独立的方法，我们研究了H-ras对转移形成的影响。分析5个在体外ras转染的10 T1/2克隆，无论是平面或平触形态显示转移潜力，H-ras的表达，和锚定非依赖性生长之间的关系。四个转移性变异来自一个转移性差，低H-ras表达线都表达高水平的H-ras RNA，并在软琼脂中有效地生长。转移瘤中H-ras表达的激活通过H-ras序列的扩增和重排而发生。此外，在NIH 3 T3系433中预诱导p21合成，显著增加了转移效率，NIH 3 T3系433含有在糖皮质激素敏感的小鼠乳腺肿瘤病毒长末端重复序列的转录控制下的v-H-ras。糖皮质激素处理正常或pEJ转化的NIH 3 T3细胞不影响转移潜力。这些数据揭示了ras表达和转移形成之间的直接关系，并表明转移和转化的表型可能在ras转化的10 T1/2和NIH 3 T3细胞中共同调节。
Using three independent approaches, we studied the effects of H-ras on metastasis formation. Analysis of five in vitro-ras-transfected 10T1/2 clones with either flat or refractile morphologies revealed a relationship between metastatic potential, H-ras expression, and anchorage-independent growth. Four metastatic variants derived from a poorly metastatic, low-H-ras-expressing line all expressed high levels of H-ras RNA and grew efficiently in soft agar. Activation of H-ras expression in the metastatic tumors had occurred through amplification and rearrangement of H-ras sequences. In addition, preinduction of p21 synthesis in NIH 3T3 line 433, which contains v-H-ras under transcriptional control of the glucocorticoid-sensitive mouse mammary tumor virus long terminal repeat, significantly increased metastatic efficiency. Glucocorticoid treatment of normal or pEJ-transformed NIH 3T3 cells did not affect metastatic potential. These data reveal a direct relationship between ras expression and metastasis formation and suggest that metastatic and transformed phenotypes may be coregulated in ras-transformed 10T1/2 and NIH 3T3 cells.
突变 c-Ha-ras 癌基因整合到 C3H/10T1/2 细胞中及其与致瘤转化的关系。
影响因子：
4.7
作者：
Manoharan,TH;Burgess,JA;Ho,D;Newell,CL;Fahl,WE
通讯作者：
Fahl,WE
肿瘤在转移部位的植入和侵袭。
DOI：
--
发表时间：
1983
期刊：
International review of experimental pathology
影响因子：
--
作者：
Nicolson,GL;Poste,G
通讯作者：
Poste,G