Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation.
Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation.
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水稻 OsUBR7 通过调节组蛋白 H2B 单泛素化和细胞增殖来调节株高
DOI：
10.1016/j.xplc.2022.100412
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发表时间：
2022-11-14
影响因子：
10.5
通讯作者：
Chu, Zhizhan
中科院分区：
文献类型：
--
作者：
Zheng, Yangyi;Zhang, Sensen;Luo, Yanqiu;Li, Fuquan;Tan, Jiantao;Wang, Bin;Zhao, Zhe;Lin, Huifang;Zhang, Tingting;Liu, Jianhong;Liu, Xupeng;Guo, Jingxin;Xie, Xianrong;Chen, Letian;Liu, Yao-Guang;Chu, Zhizhan
株高是决定水稻抗倒伏性和产量的重要农艺性状。本文报道了一个新的水稻株高相关基因OsUBR 7; OsUBR 7基因敲除导致节间细胞减少，导致半矮化表型。OsUBR 7编码一个E3连接酶，该酶含有一个植物同源结构域指和一个泛素蛋白连接酶E3组分N-recognin 7（UBR 7）结构域。OsUBR 7与组蛋白相互作用，并与E2缀合酶OsUBC 18协同作用，在赖氨酸148处单泛素化H2 B（H2 Bub 1）。OsUBR 7在许多染色质位点介导H2 Bub 1，用于靶基因的正常表达，包括细胞周期相关基因和多效性基因，这与观察结果一致，即细胞周期进程在osubr 7突变体中受到抑制，这是由于H2 Bub 1和这些位点的表达水平降低。OsUBR 7等位基因在粳稻和籼稻品种间的遗传差异影响其转录活性，这些等位基因可能在水稻驯化过程中经历了选择。总体而言，我们的研究结果揭示了一种新的机制，介导H2 Bub 1在植物中，和UBR 7直系同源物可以作为一个未开发的表观遗传资源用于作物改良。水稻UBR 7蛋白作为H2 BK 148 ub E3连接酶与E2缀合酶OsUBC 18协同发挥功能。OsUBR 7通过在多个染色质位点上介导H2 Bub 1来调节植物高度，以使目标基因（包括细胞周期相关基因和多效性基因）正常表达。UBR 7同源基因可作为一种尚未开发的表观遗传资源用于作物改良。
Plant height is an important agronomic trait for lodging resistance and yield. Here, we report a new plant-height-related gene, OsUBR7 in rice (Oryza sativa L.); knockout of OsUBR7 caused fewer cells in internodes, resulting in a semi-dwarf phenotype. OsUBR7 encodes a putative E3 ligase containing a plant homeodomain finger and a ubiquitin protein ligase E3 component N-recognin 7 (UBR7) domain. OsUBR7 interacts with histones and monoubiquitinates H2B (H2Bub1) at lysine148 in coordination with the E2 conjugase OsUBC18. OsUBR7 mediates H2Bub1 at a number of chromatin loci for the normal expression of target genes, including cell-cycle-related and pleiotropic genes, consistent with the observation that cell-cycle progression was suppressed in the osubr7 mutant owing to reductions in H2Bub1 and expression levels at these loci. The genetic divergence of OsUBR7 alleles among japonica and indica cultivars affects their transcriptional activity, and these alleles may have undergone selection during rice domestication. Overall, our results reveal a novel mechanism that mediates H2Bub1 in plants, and UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement. A rice UBR7 protein functions as an H2BK148ub E3 ligase in coordination with the E2 conjugase OsUBC18. OsUBR7 regulates plant height by mediating H2Bub1 at a number of chromatin loci for the normal expression of target genes, including cell-cycle-related and pleiotropic genes. UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement.
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