Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs.
Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs.
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DOI：
10.1002/jbm.a.32783
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Akkus, Ozan
中科院分区：
文献类型：
--
作者：
Gurkan, Umut Atakan;Cheng, Xingguo;Kishore, Vipuil;Uquillas, Jorge Alfredo;Akkus, Ozan
在美国，每年约有3300万例涉及肌肉骨骼组织（包括肌腱和韧带）的损伤。在某些情况下，肌腱和韧带受到不可逆的损伤，需要具有这些组织的天然功能特性的替代品。胶原作为一种生物材料，已成为组织工程支架的关键成分。如果能更好地控制胶原的组装过程，使其合成致密、定向的组织样结构，将大大拓宽胶原在组织工程中的应用范围。我们实验室最近开发了一种电化学方法，可以形成高度定向和密集的胶原束，其机械强度接近肌腱。然而，有有限的信息是否电化学排列的胶原束（ELAC）提出的优势，随机定向束在细胞反应方面。因此，本研究旨在评估胶原束的体外生物相容性，并比较肌腱来源的成纤维细胞（TDFs）和骨髓基质细胞（MSC）在单个和编织的ELAC束上的增殖和迁移能力。结果表明，ELAC没有细胞毒性;两种细胞类型能够更有效地在ELAC束上增殖和迁移，比随机胶原束观察到的更有效。编织的ELAC构建体在体外被TDFs和MSC有效地填充。因此，TDF和MSC都可以与ELAC束一起用于组织工程目的。
There are approximately 33 million injuries involving musculoskeletal tissues (including tendons and ligaments) every year in the United States. In certain cases the tendons and ligaments are damaged irreversibly and require replacements that possess the natural functional properties of these tissues. As a biomaterial, collagen has been a key ingredient in tissue engineering scaffolds. The application range of collagen in tissue engineering would be greatly broadened if the assembly process could be better controlled to facilitate the synthesis of dense, oriented tissue-like constructs. An electrochemical method has recently been developed in our laboratory to form highly oriented and densely packed collagen bundles with mechanical strength approaching that of tendons. However, there is limited information whether this electrochemically aligned collagen bundle (ELAC) presents advantages over randomly oriented bundles in terms of cell response. Therefore, the current study aimed to assess the biocompatibility of the collagen bundles in vitro, and compare tendon derived fibroblasts (TDFs) and bone marrow stromal cells (MSCs) in terms of their ability to populate and migrate on the single and braided ELAC bundles. The results indicated that the ELAC was not cytotoxic; both cell types were able to populate and migrate on the ELAC bundles more efficiently than that observed for random collagen bundles. The braided ELAC constructs were efficiently populated by both TDFs and MSCs in vitro. Therefore, both TDFs and MSCs can be used with the ELAC bundles for tissue engineering purposes.
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影响因子：
1.1
作者：
KRACKOW, KA;THOMAS, SC;JONES, LC
通讯作者：
JONES, LC
影响因子：
1.5
作者：
Kuo, YR;Kuo, MH;Jeng, SF
通讯作者：
Jeng, SF
DOI：
10.1016/s1095-6433(02)00247-7
发表时间：
2002-12-01
影响因子：
2.3
作者：
Koob, TJ
通讯作者：
Koob, TJ
影响因子：
14
作者：
Chen, YS;Chang, JY;Liu, BS
通讯作者：
Liu, BS
DOI：
10.1034/j.1600-0838.2000.010006312.x
发表时间：
2000-12-01
影响因子：
4.1
作者：
Kannus, P
通讯作者：
Kannus, P