The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.
The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls.
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DOI：
10.1007/s00192-018-3683-6
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发表时间：
2018-12
影响因子：
1.8
通讯作者：
Pelvic Floor Disorders Network
中科院分区：
文献类型：
--
作者：
Komesu YM;Richter HE;Carper B;Dinwiddie DL;Lukacz ES;Siddiqui NY;Sung VW;Zyczynski HM;Ridgeway B;Rogers RG;Arya LA;Mazloomdoost D;Gantz MG;Pelvic Floor Disorders Network
以前的研究表明，患有尿失禁的女性有一个改变的尿液微生物组。我们假设混合性尿失禁（MUI）女性的微生物组与对照组不同，并使用细菌基因测序技术验证了这一假设。这项多中心研究比较了MUI女性和年龄相似的对照组的尿液微生物组。获得导管尿液样本;对16 S rRNA基因的v4-6区域进行测序以识别细菌。在MUI和对照之间比较细菌优势（> 50%的个体属）。使用狄利克雷多项混合（DMM）方法将细菌序列分类为“群落类型”。广义线性混合模型根据临床特征和社区类型预测MUI/对照状态。在<51岁和≥51岁的女性中进行事后分析。样本量估计需要200个样本来检测乳杆菌优势的20%差异，P<0.05。在212份样本中，分析了97.6%（123份MUI/84份对照，平均年龄53±11岁）。总体上，MUI和对照组之间的乳酸杆菌优势没有差异（45/123=36.6% vs. 36/84= 42.9%，P=0.36）。DMM分析显示，6个社区类型;社区不同的年龄（P=0.001）。高乳酸菌（89.2%乳酸菌）社区有较大比例的控制（19/84= 22.6%，MUI 11/123=8.9%）。总体而言，细菌群落类型在MUI和对照组中没有差异。然而，对51岁以下女性的事后分析发现，细菌群落类型将MUI与对照区分开来（P=0.041）;中度乳酸菌（aOR 7.78，CI 1.85-32.62）和混合（aOR 7.10，CI 1.32-38.10）群落类型与MUI相关。在≥51岁的女性中，社区类型未区分MUI和对照组（P=0.94）。患有MUI的女性和对照组在总体乳杆菌优势方面没有差异。在年轻女性中，尿细菌群落类型将MUI与对照区分开来。
Previous studies have suggested that women with urinary incontinence have an altered urinary microbiome. We hypothesized that the microbiome in women with mixed urinary incontinence (MUI) differed from controls and tested this hypothesis using bacterial gene sequencing techniques. This multicenter study compared the urinary microbiome in women with MUI and similarly aged controls. Catheterized urine samples were obtained; v4–6 regions of the 16S rRNA gene were sequenced to identify bacteria. Bacterial predominance (> 50% of an individual’s genera) was compared between MUI and controls. Bacterial sequences were categorized into “community-types” using Dirichlet multinomial mixture (DMM) methods. Generalized linear mixed models predicted MUI/control status based on clinical characteristics and community-type. Post-hoc analyses were performed in women <51 and ≥51 years. Sample size estimates required 200 samples to detect a 20% difference in Lactobacillus predominance with P<.05. Of 212 samples, 97.6% were analyzed (123 MUI/84 controls, mean age 53±11 years). Overall Lactobacillus predominance did not differ between MUI and controls (45/123=36.6% vs. 36/84=42.9%, P=0.36). DMM analyses revealed six community-types; communities differed by age (P=0.001). A High-Lactobacillus (89.2% Lactobacillus) community had a greater proportion of controls (19/84=22.6%, MUI 11/123=8.9%). Overall, bacterial community-types did not differ in MUI and controls. However, post-hoc analysis of women <51 years found that bacterial community-types distinguished MUI from controls (P=0.041); Moderate-Lactobacillus (aOR 7.78, CI 1.85–32.62) and Mixed (aOR 7.10, CI 1.32–38.10) community-types were associated with MUI. Community-types did not differentiate MUI and controls in women ≥51 years (P=0.94). Women with MUI and controls did not differ in overall Lactobacillus predominance. In younger women, urinary bacterial community-types differentiated MUI from controls.
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影响因子：
3.7
作者：
Kumar PS;Brooker MR;Dowd SE;Camerlengo T
通讯作者：
Camerlengo T
影响因子：
1.5
作者：
Brubaker, Linda;Wolfe, Alan J.
通讯作者：
Wolfe, Alan J.
影响因子：
6.1
作者：
Arft, AM;Walker, MD;Wookey, PA
通讯作者：
Wookey, PA
DOI：
10.1016/j.ejogrb.2017.04.040
发表时间：
2017-07-01
影响因子：
2.6
作者：
Curtiss, Natasha;Balachandran, Aswini;Duckett, Jonathan
通讯作者：
Duckett, Jonathan
影响因子：
1.8
作者：
Minassian, Vatche A.;Bazi, Tony;Stewart, Walter F.
通讯作者：
Stewart, Walter F.