Simultaneous Determination of Four Active Components in Tobacco Wastes by LC
Simultaneous Determination of Four Active Components in Tobacco Wastes by LC
复制标题
液相色谱法同时测定烟草废弃物中的四种活性成分
DOI：
10.1365/s10337-008-0908-8
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发表时间：
2008-12
期刊：
影响因子：
1.7
通讯作者：
Lu, Ding-Qiang
中科院分区：
文献类型：
--
作者：
Wang, Jun;Ling, Xiu-Quan;Ouyang, Ping-Kai;Qiao, Hong-Qun;Wang, Jia-Li;Lu, Ding-Qiang
建立了一种同时测定烟草中4种主要活性成分的液相色谱分析方法。废物。样品经70%v/v甲醇水溶液提取后，采用液相色谱-电喷雾串联质谱（LC-MS/MS）和LC-UV联用技术分别鉴定和测定了绿原酸、隐绿原酸、新绿原酸和咖啡酸4种化合物。在Alltima C18柱（250 mm × 4.6 mm i.d.; 5 μm），移动的相为乙腈：乙酸铵缓冲液（pH 4.5）（5：95 v/v），流速为1.0 mL min−1，检测波长为327 nm。4个回归方程均显示各标记物的峰面积与浓度呈良好的线性关系（r2> 0.999）。该方法具有良好的重复性和精密度，保留时间和峰面积的日内和日间RSD均小于1.0%。在3个浓度水平下测得的回收率为96.33%~ 101.10%。检出限（S/N = 3）和定量限（S/N = 6）分别小于0.010和0.795 μg·mL−1。该方法成功地应用于10个样品中4种活性化合物的测定。结果表明，该方法快速、准确、可靠，可作为各种烟草废弃物的定量分析方法。
A liquid chromatographic method was developed for the simultaneous quantification of four major active components in tobacco (Nicotiana tobaccumL.) wastes. Samples were extracted with 70%v/vaqueous methanol, four compounds including chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid and caffeic acid were identified and determined by using LC coupled to electrospray tandem mass spectrometry and LC–UV method, respectively. Separation in LC–UV was on an Alltima C18column (250 mm × 4.6 mm i.d.; 5 μm) with a mobile phase consisting acetonitrile: ammonium acetate buffer (pH 4.5) (5:95v/v), at a flow rate of 1.0 mL min−1, detected at 327 nm. Four regression equations showed good linear relationships (r2> 0.999) between the peak area of each marker and concentration. The method has good repeatability and precision, the intra-day and inter-day RSD for both retention time and peak area was less than 1.0%. The recoveries, measured at three concentration levels, varied from 96.33 to 101.10%. The LOD (S/N = 3) and LOQ (S/N = 6) were less than 0.010 and 0.795 μg·mL−1, respectively. This assay was successfully applied to the determination of four active compounds in ten samples. The results indicated that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for various of tobacco wastes.
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影响因子：
2.9
作者：
C. Crofcheck;M. Loiselle;James C Weekley;I. Maiti;Sitakanta Pattanaik;P. Bummer;M. Jay
通讯作者：
C. Crofcheck;M. Loiselle;James C Weekley;I. Maiti;Sitakanta Pattanaik;P. Bummer;M. Jay
DOI：
10.1002/pca.1045
发表时间：
2008-07
期刊：
Phytochemical analysis : PCA
影响因子：
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作者：
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影响因子：
6.7
作者：
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通讯作者：
Soon Shin Kim;Chung-Kyu Lee;S. Kang;H. Jung;J. Choi
DOI：
10.1016/j.chroma.2007.01.065
发表时间：
2007-03
期刊：
Journal of chromatography. A
影响因子：
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作者：
Xueling Du;Qipeng Yuan;Jinsong Zhao;Ye Li
通讯作者：
Xueling Du;Qipeng Yuan;Jinsong Zhao;Ye Li
DOI：
10.1016/s1572-5995(01)80024-7
发表时间：
2001
期刊：
Studies in natural products chemistry
影响因子：
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作者：
H. Morishita;M. Ohnishi
通讯作者：
H. Morishita;M. Ohnishi