A microfluidic interface for the culture and sampling of adiponectin from primary adipocytes.
A microfluidic interface for the culture and sampling of adiponectin from primary adipocytes.
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DOI：
10.1039/c4an01725k
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发表时间：
2015-02-21
期刊：
影响因子：
--
通讯作者：
Easley CJ
中科院分区：
文献类型：
--
作者：
Godwin LA;Brooks JC;Hoepfner LD;Wanders D;Judd RL;Easley CJ
脂联素从脂肪组织中分泌，是一种重要的内分泌激素，作用于葡萄糖代谢，从而在糖尿病、肥胖和其他代谢疾病状态中发挥关键作用。胰岛素暴露于在静态条件下培养的原代脂肪细胞已被证明刺激脂联素分泌。然而，用于培养和刺激脂肪细胞的常规静态方法福尔斯达不到真正模拟生理环境。沿着实验成本和样品体积的降低以及时间分辨率的增加，微流体平台允许小体积流动的细胞培养系统，其更准确地表示通过体内脉管系统的恒定流动条件。在这里，我们已经将定制的原代组织培养储器集成到由聚二甲基硅氧烷（PDMS）制成的被动操作的微流体装置中。通过采样通道上方独特的PDMS“美化”完成储库的制造，其中设计策略针对原代脂肪细胞以克服正细胞浮力的问题。该储库允许原代鼠脂肪细胞的三维培养，通过恒定灌注精确控制刺激物，以及在各种治疗期间对脂肪因子分泌进行采样。作为微流控系统内原代脂肪细胞培养和采样的第一份报告，这项工作为脂肪因子分泌动力学的未来研究奠定了基础。
Secreted from adipose tissue, adiponectin is a vital endocrine hormone that acts in glucose metabolism, thereby establishing its crucial role in diabetes, obesity, and other metabolic disease states. Insulin exposure to primary adipocytes cultured in static conditions has been shown to stimulate adiponectin secretion. However, conventional, static methodology for culturing and stimulating adipocytes falls short of truly mimicking physiological environments. Along with decreases in experimental costs and sample volume, and increased temporal resolution, microfluidic platforms permit small-volume flowing cell culture systems, which more accurately represent the constant flow conditions through vasculature in vivo. Here, we have integrated a customized primary tissue culture reservoir into a passively operated microfluidic device made of polydimethylsiloxane (PDMS). Fabrication of the reservoir was accomplished through unique PDMS “landscaping” above sampling channels, with a design strategy targeted to primary adipocytes to overcome issues of positive cell buoyancy. This reservoir allowed three-dimensional culture of primary murine adipocytes, accurate control over stimulants via constant perfusion, and sampling of adipokine secretion during various treatments. As the first report of primary adipocyte culture and sampling within microfluidic systems, this work sets the stage for future studies in adipokine secretion dynamics.
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影响因子：
7.4
作者：
Dishinger, John F.;Reid, Kendra R.;Kennedy, Robert T.
通讯作者：
Kennedy, Robert T.
影响因子：
6.9
作者：
Ahima, Rexford S.
通讯作者：
Ahima, Rexford S.
用于研究药物输送系统的器官片平台。
DOI：
10.1016/j.jconrel.2014.05.004
发表时间：
2014-09-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Bhise NS;Ribas J;Manoharan V;Zhang YS;Polini A;Massa S;Dokmeci MR;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
46.9
作者：
Bhatia, Sangeeta N.;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
影响因子：
6.1
作者：
Kim, Sudong;Lee, Hyunjae;Jeon, Noo Li
通讯作者：
Jeon, Noo Li