Glucose 6P binds and activates HlyIIR to repress Bacillus cereus haemolysin hlyII gene expression.
Glucose 6P binds and activates HlyIIR to repress Bacillus cereus haemolysin hlyII gene expression.
复制标题
DOI：
10.1371/journal.pone.0055085
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ramarao N
中科院分区：
文献类型：
--
作者：
Guillemet E;Tran SL;Cadot C;Rognan D;Lereclus D;Ramarao N
蜡样芽孢杆菌是一种革兰氏阳性孢子形成细菌，可引起食物中毒和严重的机会性感染。这些感染的特征是细菌积聚，尽管吞噬细胞的募集。我们之前已经证明了B。蜡状溶血素II（HlyII）通过凋亡诱导巨噬细胞死亡。在这项工作中，我们研究了hlyII基因的调控。我们发现，HlyIIR，hlyII表达的负调节剂在B。蜡状芽孢杆菌在早期细菌生长阶段特别活跃。我们证明，葡萄糖6P直接结合到HlyIIR和增强其活性在转录后水平。葡萄糖6P激活HlyIIR，增加其与位于hlyII基因上游的DNA盒结合的能力，抑制其表达。因此，hlyII表达受葡萄糖的可用性调节。由于HlyII诱导血细胞和巨噬细胞死亡，这两种细胞类型在感染后的营养物隔离中起作用，HlyII可能诱导宿主细胞死亡，以允许细菌获得作为细菌生长必需组分的碳源。
Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. We have previously shown that B. cereus Haemolysin II (HlyII) induces macrophage cell death by apoptosis. In this work, we investigated the regulation of the hlyII gene. We show that HlyIIR, the negative regulator of hlyII expression in B. cereus, is especially active during the early bacterial growth phase. We demonstrate that glucose 6P directly binds to HlyIIR and enhances its activity at a post-transcriptional level. Glucose 6P activates HlyIIR, increasing its capacity to bind to its DNA-box located upstream of the hlyII gene, inhibiting its expression. Thus, hlyII expression is modulated by the availability of glucose. As HlyII induces haemocyte and macrophage death, two cell types that play a role in the sequestration of nutrients upon infection, HlyII may induce host cell death to allow the bacteria to gain access to carbon sources that are essential components for bacterial growth.
登录
查看更多内容
影响因子：
2.8
作者：
Harvie, DR;Vílchez, S;Ellar, DJ
通讯作者：
Ellar, DJ
影响因子：
1.2
作者：
Arnaout, MK;Tamburro, RF;Ribeiro, PC
通讯作者：
Ribeiro, PC
影响因子：
2.1
作者：
Baida, G;Budarina, ZI;Solonin, AS
通讯作者：
Solonin, AS
影响因子：
9.4
作者：
Cadot, Celine;Tran, Seav-Ly;Ramarao, Nalini
通讯作者：
Ramarao, Nalini
影响因子：
4.4
作者：
Bouillaut, L;Ramarao, N;Nielsen-LeRoux, C
通讯作者：
Nielsen-LeRoux, C