Osteogenic potential of human adipose derived stem cells (hASCs) seeded on titanium trabecular spinal cages.
Osteogenic potential of human adipose derived stem cells (hASCs) seeded on titanium trabecular spinal cages.
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人脂肪衍生的干细胞（HASC）的成骨潜力，属于小梁钛脊柱笼子。
DOI：
10.1038/s41598-020-75385-y
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发表时间：
2020-10-26
影响因子：
4.6
通讯作者：
Gastaldi G
中科院分区：
文献类型：
--
作者：
Caliogna L;Bina V;Botta L;Benazzo FM;Medetti M;Maestretti G;Mosconi M;Cofano F;Tartara F;Gastaldi G
脊柱退行性疾病越来越普遍，影响了欧洲约 5.7% 的人口，导致生活质量显着下降。到目前为止，许多材料已被用来制造笼状植入物，用作移植物替代品，以实现即时和长期的脊柱固定。特别是，钛及其合金正在成为开发新型保持架的重要候选者。这项体外研究的目的是评估接种在小梁钛笼上的脂肪来源间充质干细胞（ASC）的粘附、增殖和成骨分化。在 3 周的培养过程中，ASC 粘附、增殖并产生丰富的细胞外基质。在成骨培养基存在的情况下，ASC 分化为成骨细胞样细胞：典型骨基因的表达以及碱性磷酸酶活性在统计学上高于对照组。此外，色散光谱微量分析显示，在成骨培养基中生长的细胞中钙水平显着增加。另外，我们关于骨诱导的初步数据表明，这种钛植入物有可能诱导 ASC 产生分泌组，从而触发 ASC 表型的转变，可能向成骨分化方向发展，如 qRT-PCR 和 ALP 生化测定结果所示。这些笼子的小梁多孔组织与松质骨结构非常相似，因此允许骨基质有效地定殖在其上；由于这些原因，我们可以得出结论，该笼的结构可能在调节植入物的骨诱导能力方面发挥作用，从而鼓励其参与治疗脊柱畸形和疾病的体内研究。
Spine degenerative conditions are becoming increasingly prevalent, affecting about 5.7% of the population in Europe, resulting in a significant reduction of life’s quality. Up to now, many materials have been used in manufacturing cage implants, used as graft substitutes, to achieve immediate and long-term spinal fixation. Particularly, titanium and its alloys are emerging as valuable candidates to develop new types of cages. The aim of this in vitro study was to evaluate the adhesion, proliferation and osteogenic differentiation of adipose derived mesenchymal stem cells (ASCs) seeded on trabecular titanium cages. ASCs adhered, proliferated and produced an abundant extracellular matrix during the 3 weeks of culture. In the presence of osteogenic medium, ASCs differentiated into osteoblast-like cells: the expression of typical bone genes, as well as the alkaline phosphatase activity, was statistically higher than in controls. Furthermore, the dispersive spectrometry microanalysis showed a marked increase of calcium level in cells grown in osteogenic medium. Plus, our preliminary data about osteoinduction suggest that this titanium implant has the potential to induce the ASCs to produce a secretome able to trigger a shift in the ASCs phenotype, possibly towards the osteogenic differentiation, as illustrated by the qRT-PCR and ALP biochemical assay results. The trabecular porous organization of these cages is rather similar to the cancellous bone structure, thus allowing the bone matrix to colonize it efficiently; for these reasons we can conclude that the architecture of this cage may play a role in modulating the osteoinductive capabilities of the implant, thus encouraging its engagement in in vivo studies for the treatment of spinal deformities and diseases.
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影响因子：
3
作者：
McConnell, JR;Freeman, BJC;Webb, JK
通讯作者：
Webb, JK
影响因子：
4.1
作者：
Hacker, RJ
通讯作者：
Hacker, RJ
影响因子：
3
作者：
Fountas, Kostas N.;Kapsalaki, Eftychia Z.;Robinson, Joe S.
通讯作者：
Robinson, Joe S.
影响因子：
2.8
作者：
Matsumoto, Tomiya;Okuda, Shinya;Iwasaki, Motoki
通讯作者：
Iwasaki, Motoki
影响因子：
3.7
作者：
Linero I;Chaparro O
通讯作者：
Chaparro O