Defeating Antibiotic- and Phage-Resistant Enterococcus faecalis Using a Phage Cocktail in Vitro and in a Clot Model.
Defeating Antibiotic- and Phage-Resistant Enterococcus faecalis Using a Phage Cocktail in Vitro and in a Clot Model.
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DOI：
10.3389/fmicb.2018.00326
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发表时间：
2018
影响因子：
5.2
通讯作者：
Hazan R
中科院分区：
文献类型：
--
作者：
Khalifa L;Gelman D;Shlezinger M;Dessal AL;Coppenhagen-Glazer S;Beyth N;Hazan R
抗生素疗效的恶化促使全世界的研究人员转向噬菌体疗法等替代技术：使用称为噬菌体的细菌病毒治疗传染病。在先前的论文中，我们分离出了噬菌体EFDG1，它对最具挑战性的致病性菌种之一万古霉素耐药肠球菌（VRE）的增殖和生物膜培养都非常有效。因此，它是一种很有希望用于噬菌体治疗的噬菌体。进一步的实验揭示了对EFDG1噬菌体具有抗性的突变体的出现：EFDG1r。这种对抗生素的自发耐药性将是灾难性的，然而对于噬菌体治疗来说，它只是一个小障碍。我们快速成功地分离出一种新的噬菌体EFLK1，它被证明对耐药突变体EFDG1r及其亲本VRE粪肠球菌V583有效。此外，在混合物中结合两种药物产生了抗E. faecalis V583菌株，而不管它们的抗生素或噬菌体抗性谱。分析的差异，基因组序列，基因，突变，和tRNA含量的两个ESTA。这项工作是噬菌体疗法最重要优势之一的概念验证，即能够轻松克服新出现的耐药细菌。
The deteriorating effectiveness of antibiotics is propelling researchers worldwide towards alternative techniques such as phage therapy: curing infectious diseases using viruses of bacteria called bacteriophages. In a previous paper, we isolated phage EFDG1, highly effective against both planktonic and biofilm cultures of one of the most challenging pathogenic species, the vancomycin-resistant Enterococcus (VRE). Thus, it is a promising phage to be used in phage therapy. Further experimentation revealed the emergence of a mutant resistant to EFDG1 phage: EFDG1r. This kind of spontaneous resistance to antibiotics would be disastrous occurrence, however for phage-therapy it is only a minor hindrance. We quickly and successfully isolated a new phage, EFLK1, which proved effective against both the resistant mutant EFDG1r and its parental VRE, Enterococcus faecalis V583. Furthermore, combining both phages in a cocktail produced an additive effect against E. faecalis V583 strains regardless of their antibiotic or phage-resistance profile. An analysis of the differences in genome sequence, genes, mutations, and tRNA content of both phages is presented. This work is a proof-of-concept of one of the most significant advantages of phage therapy, namely the ability to easily overcome emerging resistant bacteria.
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影响因子：
3.7
作者：
Andrew AS;Hu T;Gu J;Gui J;Ye Y;Marsit CJ;Kelsey KT;Schned AR;Tanyos SA;Pendleton EM;Mason RA;Morlock EV;Zens MS;Li Z;Moore JH;Wu X;Karagas MR
通讯作者：
Karagas MR
影响因子：
11.4
作者：
Charles, PGP;Grayson, ML
通讯作者：
Grayson, ML
影响因子：
2.7
作者：
BERTRAM, H
通讯作者：
BERTRAM, H
影响因子：
5.6
作者：
Krishnan, Sriram Tiruvadi;Moolman, M. Charl;Dekker, Nynke H.
通讯作者：
Dekker, Nynke H.
影响因子：
11.8
作者：
Donlan, R M
通讯作者：
Donlan, R M