Integrated Analysis of Genome-Wide Copy Number Alterations and Gene Expression Profiling of Lung Cancer in Xuanwei, China.
Integrated Analysis of Genome-Wide Copy Number Alterations and Gene Expression Profiling of Lung Cancer in Xuanwei, China.
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中国宣威市肺癌全基因组拷贝数改变和基因表达谱的综合分析
DOI：
10.1371/journal.pone.0169098
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Xue Q;Pan G;Meng QH;Tuo X;Cai X;Chen Z;Li Y;Huang T;Duan X;Duan Y
中国宣威肺癌以其独特的特征而闻名于世，但其发病机制却知之甚少。本研究旨在筛选LCXW中潜在的新“驱动基因”。通过基于阵列的比较基因组杂交检测8对LCXW和非癌肺组织中全基因组DNA拷贝数变异（CNA），并通过基因表达微阵列检测差异表达基因（DEG）。通过CNA和DEG的综合分析筛选候选驱动基因。通过实时定量聚合酶链反应进一步验证候选基因。分别检测到大量CNA和DEG。一些最常见的CNA包括5p15.33-p15.32、5p15.1-p14.3和5p14.3-p14.2的增加以及11q24.3、21q21.1、21q22.12-q22.13和21q22.2的丢失。CNA和DEG的综合分析确定了24个具有频繁拷贝数增加和一致上调的候选基因，这些候选基因被认为是潜在的癌基因，包括CREB 3L 4、TRIP 13和CCNE 2。此外，该分析确定了19个候选基因，其拷贝数变化与表达变化之间存在负相关性，被认为是潜在的肿瘤抑制基因，包括AHRR，NKD 2和KLF 10。研究最多的致癌基因之一，MYC，可能不会在LCXW中发挥致癌作用。CNA和DEG的综合分析确定了几个潜在的新的LCXW相关基因，为进一步研究LCXW的发病机制和识别新的生物标志物或治疗靶点奠定了重要基础。
Lung cancer in Xuanwei (LCXW), China, is known throughout the world for its distinctive characteristics, but little is known about its pathogenesis. The purpose of this study was to screen potential novel “driver genes” in LCXW. Genome-wide DNA copy number alterations (CNAs) were detected by array-based comparative genomic hybridization and differentially expressed genes (DEGs) by gene expression microarrays in 8 paired LCXW and non-cancerous lung tissues. Candidate driver genes were screened by integrated analysis of CNAs and DEGs. The candidate genes were further validated by real-time quantitative polymerase chain reaction. Large numbers of CNAs and DEGs were detected, respectively. Some of the most frequently occurring CNAs included gains at 5p15.33-p15.32, 5p15.1-p14.3, and 5p14.3-p14.2 and losses at 11q24.3, 21q21.1, 21q22.12-q22.13, and 21q22.2. Integrated analysis of CNAs and DEGs identified 24 candidate genes with frequent copy number gains and concordant upregulation, which were considered potential oncogenes, including CREB3L4, TRIP13, and CCNE2. In addition, the analysis identified 19 candidate genes with a negative association between copy number change and expression change, considered potential tumor suppressor genes, including AHRR, NKD2, and KLF10. One of the most studied oncogenes, MYC, may not play a carcinogenic role in LCXW. This integrated analysis of CNAs and DEGs identified several potential novel LCXW-related genes, laying an important foundation for further research on the pathogenesis of LCXW and identification of novel biomarkers or therapeutic targets.
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影响因子：
37.3
作者：
Hu T;Li C
通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Natkunam, Yasodha
DOI：
10.1080/00039896.1988.9935850
发表时间：
1988-03-01
期刊：
ARCHIVES OF ENVIRONMENTAL HEALTH
影响因子：
--
作者：
CHAPMAN, RS;MUMFORD, JL;YANG, RD
通讯作者：
YANG, RD
影响因子：
1.7
作者：
Sarholz, Barbara;Piepho, Hans-Peter
通讯作者：
Piepho, Hans-Peter