FINE CULM1 (FC1) works downstream of strigolactones to inhibit the outgrowth of axillary buds in rice.
FINE CULM1 (FC1) works downstream of strigolactones to inhibit the outgrowth of axillary buds in rice.
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DOI：
10.1093/pcp/pcq083
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发表时间：
2010-07
影响因子：
4.9
通讯作者：
Kyozuka J
中科院分区：
文献类型：
--
作者：
Minakuchi K;Kameoka H;Yasuno N;Umehara M;Luo L;Kobayashi K;Hanada A;Ueno K;Asami T;Yamaguchi S;Kyozuka J
最近对豌豆、拟南芥和水稻的高度分枝突变体的研究表明，独脚金内酯（SL）作为抑制芽分枝的激素。为了更好地了解SL如何控制腋芽的生长，需要鉴定SL下游的基因。我们发现，水稻细茎1（fc 1）突变体增加的分蘖表型不能通过施用1 μM GR 24（一种合成的SL类似物）来挽救。高浓度GR 24（10 μM）处理可抑制野生型植株的分蘖生长，但对fc 1突变体无效，这意味着SL抑制芽生长需要适当的FC 1功能。FC 1的过表达部分挽救了分蘖生长和株高的d3-2缺陷。原位杂交分析表明，FC 1 mRNA在腋芽、茎尖分生组织、幼叶、维管组织和冠状根尖中积累。FC 1 mRNA表达没有显着影响GR 24，这表明转录诱导可能不是SL影响FC 1功能的机制。另一方面，FC 1的表达水平受到细胞分裂素处理的负调控。我们建议，FC 1作为一个集成器的多个信号通路，是必不可少的微调在水稻芽分枝。
Recent studies of highly branched mutants of pea, Arabidopsis and rice have demonstrated that strigolactones (SLs) act as hormones that inhibit shoot branching. The identification of genes that work downstream of SLs is required for a better understanding of how SLs control the growth of axillary buds. We found that the increased tillering phenotype of fine culm1 (fc1) mutants of rice is not rescued by the application of 1 μM GR24, a synthetic SL analog. Treatment with a high concentration of GR24 (10 μM) causes suppression of tiller growth in wild-type plants, but is not effective on fc1 mutants, implying that proper FC1 functioning is required for SLs to inhibit bud growth. Overexpression of FC1 partially rescued d3-2 defects in the tiller growth and plant height. An in situ hybridization analysis showed that FC1 mRNA accumulates in axillary buds, the shoot apical meristem, young leaves, vascular tissues and the tips of crown roots. FC1 mRNA expression was not significantly affected by GR24, suggesting that transcriptional induction may not be the mechanism by which SLs affect FC1 functioning. On the other hand, the expression level of FC1 is negatively regulated by cytokinin treatment. We propose that FC1 acts as an integrator of multiple signaling pathways and is essential to the fine-tuning of shoot branching in rice.
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