Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma.
Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma.
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DOI：
10.1016/j.jaci.2010.10.048
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发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
中科院分区：
文献类型：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
大环内酯类抗生素治疗后肺功能的改善归因于特定细菌引起的支气管感染减少。然而，气道可能存在更多样化的微生物群，哮喘的临床特征可能与存在的气道微生物群的特征有关。
为了确定气道细菌微生物群的组成与哮喘临床特征之间是否存在关系，使用不依赖培养的工具，这些工具能够检测大多数已知细菌的存在和相对丰度。
在这项初步研究中，从65名哮喘控制欠佳的成年人（他们参与了一项关于克拉霉素对哮喘控制影响的多中心研究）以及10名健康受试者中收集了支气管上皮刷检样本。采用高密度16S rRNA微阵列和平行克隆文库测序分析相结合的方法来分析微生物群，并研究其与临床测量指标的关系。
与对照组相比，哮喘患者的16S rRNA扩增子浓度（细菌负荷的一个指标）和细菌多样性显著更高。在多变量分析中，气道微生物群的组成和多样性与支气管高反应性显著相关。具体而言，特定分类群的相对丰度，包括丛毛单胞菌科、鞘氨醇单胞菌科、草酸杆菌科和其他细菌家族的成员，与支气管高反应性的程度高度相关。
支气管气道微生物群的组成与哮喘控制欠佳患者的支气管高反应性程度相关。这些发现支持有必要进行进一步的功能研究，以检验气道微生物群成员在哮喘发病机制中的潜在作用。
Improvement in lung function following macrolide antibiotic therapy has been attributed to reduction in bronchial infection due to specific bacteria. However, the airway may be populated by a more diverse microbiota, and clinical features of asthma may be associated with characteristics of the airway microbiota present. To determine if relationships exist between the composition of the airway bacterial microbiota and clinical features of asthma, using culture-independent tools capable of detecting the presence and relative abundance of most known bacteria. In this pilot study, bronchial epithelial brushings were collected from sixty-five adults with sub-optimally controlled asthma participating in a multicenter study of the effects of clarithromycin on asthma control, and ten healthy subjects. A combination of high-density 16S rRNA microarray and parallel clone library-sequencing analysis was used to profile the microbiota and examine relationships with clinical measurements. Compared to controls, 16S rRNA amplicon concentrations (a proxy for bacterial burden) and bacterial diversity were significantly higher among asthmatic patients. In multivariate analyses, airway microbiota composition and diversity were significantly correlated with bronchial hyperresponsiveness. Specifically, the relative abundance of particular phylotypes, including members of the Comamonadaceae, Sphingomonadaceae, Oxalobacteraceae and other bacterial families, were highly correlated with the degree of bronchial hyperresponsiveness. The composition of bronchial airway microbiota is associated with the degree of bronchial hyperresponsiveness among patients with sub-optimally controlled asthma. These findings support the need for further functional studies to examine the potential contribution of members of the airway microbiota in asthma pathogenesis.
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DOI：
10.1073/pnas.0608255104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Brodie, Eoin L.;DeSantis, Todd Z.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
2.1
作者：
Einen, Jorn;Thorseth, Ingunn H.;Ovreas, Lise
通讯作者：
Ovreas, Lise
影响因子：
9.4
作者：
Flanagan, J. L.;Brodie, E. L.;Bristow, J.
通讯作者：
Bristow, J.
影响因子：
158.5
作者：
Bisgaard, Hans;Hermansen, Mette Northman;Pipper, Christian Bressen
通讯作者：
Pipper, Christian Bressen
DOI：
10.1164/ajrccm.164.4.2011040
发表时间：
2001-08-15
影响因子：
24.7
作者：
Black, PN;Blasi, F;Allegra, L
通讯作者：
Allegra, L