Irritable Bowel Syndrome, Particularly the Constipation-Predominant Form, Involves an Increase in Methanobrevibacter smithii, Which Is Associated with Higher Methane Production.
Irritable Bowel Syndrome, Particularly the Constipation-Predominant Form, Involves an Increase in Methanobrevibacter smithii, Which Is Associated with Higher Methane Production.
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DOI：
10.5009/gnl15588
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发表时间：
2016-11-15
期刊：
影响因子：
3.4
通讯作者：
Ghoshal UC
中科院分区：
文献类型：
--
作者：
Ghoshal U;Shukla R;Srivastava D;Ghoshal UC
由于史氏甲烷短杆菌产生甲烷，延迟肠道运输，我们评估了M。肠易激综合征（IBS）患者和健康对照（HC）中的史密氏菌负荷。实时定量聚合酶链反应检测M.对47名IBS患者（罗马III）和30名HC的粪便进行Smithii。在乳果糖氢呼气试验（LHBT，对25名IBS患者进行）中，使用10 g乳果糖的空腹甲烷结果≥10 ppm定义为甲烷产生者。47例中，20例便秘（IBS-C），20例腹泻（IBS-D），7例未分型。分枝IBS患者的smithii拷贝数高于HC患者（Log105.4，四分位距[IQR; 3.2 - 6.3] vs 1.9 [0.0 - 3.4]，p<0.001），尤其是IBS-C患者与IBS-D患者相比（Log106.1 [5.5 - 6.6] vs 3.4 [0.6 - 5.7]，p=0.001）;拷贝数与大便频率呈负相关（R=-0.420，p=0.003）。分枝产甲烷者的smithii拷贝数高于非产甲烷者（Log106.4，IQR [5.7 - 7.4] vs 4.1 [1.8 - 5.8]，p=0.001）。使用受试者工作特征曲线，M。Smithii在甲烷产生者中的平均值为Log106.0（灵敏度，64%;特异性，86%;曲线下面积[AUC]，0.896）。呼气甲烷的AUC与M. Smithii基因拷贝数在甲烷产生者中的分布（r=0.74，p=0.008）。腹胀在甲烷产生者中更常见（n=9/11 [82%] vs 5/14 [36%]，p=0.021）。IBS患者，尤其是IBS-C患者，M基因拷贝数较高。史密斯比HC。在LHBT上，呼吸甲烷水平与M。史密斯装载。
Because Methanobrevibacter smithii produces methane, delaying gut transit, we evaluated M. smithii loads in irritable bowel syndrome (IBS) patients and healthy controls (HC). Quantitative real-time polymerase chain reaction for M. smithii was performed on the feces of 47 IBS patients (Rome III) and 30 HC. On the lactulose hydrogen breath test (LHBT, done for 25 IBS patients), a fasting methane result ≥10 ppm using 10 g of lactulose defined methane-producers. Of 47, 20 had constipation (IBS-C), 20 had diarrhea (IBS-D) and seven were not sub-typed. The M. smithii copy number was higher among IBS patients than HC (Log105.4, interquartile range [IQR; 3.2 to 6.3] vs 1.9 [0.0 to 3.4], p<0.001), particularly among IBS-C compared to IBS-D patients (Log106.1 [5.5 to 6.6] vs 3.4 [0.6 to 5.7], p=0.001); the copy number negatively correlated with the stool frequency (R=−0.420, p=0.003). The M. smithii copy number was higher among methane-producers than nonproducers (Log106.4, IQR [5.7 to 7.4] vs 4.1 [1.8 to 5.8], p=0.001). Using a receiver operating characteristic curve, the best cutoff for M. smithii among methane producers was Log106.0 (sensitivity, 64%; specificity, 86%; area under curve [AUC], 0.896). The AUC for breath methane correlated with the M. smithii copy number among methane producers (r=0.74, p=0.008). Abdominal bloating was more common among methane producers (n=9/11 [82%] vs 5/14 [36%], p=0.021). Patients with IBS, particularly IBS-C, had higher copy numbers of M. smithii than HC. On LHBT, breath methane levels correlated with M. smithii loads.
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影响因子：
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作者：
Ghoshal UC;Shukla R;Ghoshal U;Gwee KA;Ng SC;Quigley EM
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