Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes.
Selection on oxidative phosphorylation and ribosomal structure as a multigenerational response to ocean acidification in the common copepod Pseudocalanus acuspes.
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DOI：
10.1111/eva.12335
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发表时间：
2016-10
影响因子：
4.1
通讯作者：
Thor P
中科院分区：
文献类型：
--
作者：
De Wit P;Dupont S;Thor P
海洋酸化预计将对海洋生态系统产生巨大影响，但令人惊讶的是，目前很少有研究考察海洋无脊椎动物对pCO 2压力的长期适应性和可塑性反应。本文将尖突拟哲水蚤（Pseudocalanus acuspes）暴露于pCO2浓度分别为400、900和1550 μatm的环境中两代，然后进行交互移植实验。组装并注释了从头转录组，基因表达数据显示，参与RNA转录的基因在长期暴露于高pCO 2环境的人群中强烈下调，即使在移植后恢复到对照水平。另外，共检测到747000个SNPs，其中1513个SNPs的核苷酸频率在对照组和高pCO2组之间变化一致。涉及RNA转录和核糖体功能，以及离子转运和氧化磷酸化的功能，是高度过度。因此，我们得出结论，二氧化碳压力似乎施加选择桡足类RNA的合成和翻译，可能调制解旋酶的表达。使用生理假设检验策略来挖掘基因表达数据，我们在此增加了检测海洋酸化细胞目标的能力。这种新的方法似乎有希望为未来的研究环境变化的影响，在生态上重要的非模式生物。
Ocean acidification is expected to have dramatic impacts on oceanic ecosystems, yet surprisingly few studies currently examine long‐term adaptive and plastic responses of marine invertebrates to pCO 2 stress. Here, we exposed populations of the common copepod Pseudocalanus acuspes to three pCO 2 regimes (400, 900, and 1550 μatm) for two generations, after which we conducted a reciprocal transplant experiment. A de novo transcriptome was assembled, annotated, and gene expression data revealed that genes involved in RNA transcription were strongly down‐regulated in populations with long‐term exposure to a high pCO 2 environment, even after transplantation back to control levels. In addition, 747 000 SNPs were identified, out of which 1513 showed consistent changes in nucleotide frequency between replicates of control and high pCO 2 populations. Functions involving RNA transcription and ribosomal function, as well as ion transport and oxidative phosphorylation, were highly overrepresented. We thus conclude that pCO 2 stress appears to impose selection in copepods on RNA synthesis and translation, possibly modulated by helicase expression. Using a physiological hypothesis‐testing strategy to mine gene expression data, we herein increase the power to detect cellular targets of ocean acidification. This novel approach seems promising for future studies of effects of environmental changes in ecologically important nonmodel organisms.
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影响因子：
64.8
作者：
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通讯作者：
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DOI：
10.1016/j.jembe.2015.09.016
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