The Expression of Chemosensory Genes in Male Maxillary Palps of Anopheles coluzzii (Diptera: Culicidae) and An. quadriannulatus.
The Expression of Chemosensory Genes in Male Maxillary Palps of Anopheles coluzzii (Diptera: Culicidae) and An. quadriannulatus.
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DOI：
10.1093/jme/tjaa290
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发表时间：
2021-05-15
影响因子：
2.1
通讯作者：
Slotman MA
中科院分区：
文献类型：
--
作者：
Athrey G;Popkin-Hall ZR;Takken W;Slotman MA
由于其作为疟疾媒介的重要性，科洛兹按蚊的Coetzee & Wilkerson嗅觉系统已被广泛研究。在这些工作中，有一系列的研究比较了这些物种的雌性和/或雄性嗅觉器官中化学感觉基因的表达。这些研究已经确定了物种和雌性偏向的化学感觉基因表达模式。然而，化学感觉在雄性按蚊生物学中的作用仍然存在许多问题。为了为今后的工作铺平道路，我们利用RNAseq比较了雄性安家鸡上颌触须的化学感觉基因表达。coluzzii和它的兄弟物种An。quadriannulatus西奥博尔德。正如预期的那样，化学感觉基因库在雄性上颌触须中很小。两种植物的调节受体Or8和Or28的表达水平均较高。二氧化碳受体基因Gr22-Gr24也存在于这两个物种中，尽管比雌性低得多。此外，一些化学感受器是物种特异性的。Gr37和Gr52是An所独有的。而Or9和Gr60仅在An中检测到。quadriannulatus。此外，一些化学感觉基因在两个物种之间表现出差异表达。最后，一些在雌性触须中表现出强烈差异表达的Irs、Grs和Obps在雄性触须中缺失或低表达。虽然化学感觉在按蚊雄性生物学中的作用仍然存在许多问题，但这些结果表明，雄性上颌触须在化学刺激的感知中可能具有性别和物种特异性的作用。这项工作对进一步研究雄性上颌触须在这些物种中的作用具有指导意义。
Because of its importance as a malaria vector, Anopheles coluzzii’s Coetzee & Wilkerson olfactory system has been studied extensively. Among this work is a series of studies comparing the expression of chemosensory genes in olfactory organs in females and/or males of these species. These have identified species- and female-biased chemosensory gene expression patterns. However, many questions remain about the role of chemosensation in male anopheline biology. To pave the way for future work we used RNAseq to compare chemosensory gene expression in the male maxillary palps of An. coluzzii and its sibling species An. quadriannulatus Theobald. As expected, the chemosensory gene repertoire is small in the male maxillary palps. Both species express the tuning receptors Or8 and Or28 at relatively high levels. The CO2 receptor genes Gr22-Gr24 are present in both species as well, although at much lower level than in females. Additionally, several chemoreceptors are species-specific. Gr37 and Gr52 are exclusive to An. coluzzii, whereas Or9 and Gr60 were detected only in An. quadriannulatus. Furthermore, several chemosensory genes show differential expression between the two species. Finally, several Irs, Grs, and Obps that show strong differential expression in the female palps, are absent or lowly expressed in the male palps. While many questions remain about the role of chemosensation in anopheline male biology, these results suggest that the male maxillary palps could have both a sex- and species-specific role in the perception of chemical stimuli. This work may guide future studies on the role of the male maxillary palp in these species.
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影响因子：
1.9
作者：
HEALY, TP;JEPSON, PC
通讯作者：
JEPSON, PC
进化保守的气味受体功能质疑八烯醇在蚊子中作用的生态环境。
影响因子：
4.6
作者：
Dekel A;Pitts RJ;Yakir E;Bohbot JD
通讯作者：
Bohbot JD
DOI：
10.1007/bf00187475
发表时间：
1995-10-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
GRANT, AJ;WIGTON, BE;OCONNELL, RJ
通讯作者：
OCONNELL, RJ
DOI：
10.1073/pnas.251532998
发表时间：
2001-12-04
影响因子：
11.1
作者：
Horst, R;Damberger, F;Wüthrich, K
通讯作者：
Wüthrich, K
DOI：
10.2987/8756-971x(2007)23
发表时间：
2007-01-01
影响因子：
1
作者：
Platzer, Edward G. -
通讯作者：
Platzer, Edward G. -