Purification, characterization, and partial amino acid sequence of nerve growth factor from cobra venom.
Purification, characterization, and partial amino acid sequence of nerve growth factor from cobra venom.
复制标题
眼镜蛇毒液中神经生长因子的纯化、表征和部分氨基酸序列。
作者：
R. Hogue;W. Frazier;J. Jacobs;H. Niall;R. Bradshaw
从眼镜蛇毒中分离纯化神经生长因子（NGF），并与小鼠颌下腺中的NGF进行结构比较。设计了两步纯化程序，包括在1 M乙酸中的凝胶过滤步骤，然后在pH 5的羧甲基纤维素上对活性合并液进行色谱分析。发现天然蛋白质的分子量为28000，并且该值在变性条件下减少约一半。这些值与小鼠2.5S或β NGF获得的值相当。S-[14 C]羧甲基神经生长因子的胰蛋白酶肽图谱给出了由两个相同或非常相似的亚基组成的结构所预期的标记肽的数量。因此，小鼠和眼镜蛇NGF的四级结构相同。溴化氰（CNBr）处理的眼镜蛇神经生长因子产生三个片段，其中两个被纯化到同质。在自动蛋白测序仪中分析这些片段和整个蛋白。氨基末端的CNBr片段的蛋白质也进行消化嗜热菌蛋白酶和所得的肽进行纯化和表征。这些数据加上那些从胰蛋白酶肽的表征提供了一个初步的一级结构的眼镜蛇神经生长因子，这是约60%的相同，与小鼠神经生长因子的建设的基础。
The nerve growth factor (NGF) from Naja naja (cobra) venom has been purified and its structure compared to the NGF from mouse submaxillary gland. A two-step purification procedure has been devised, consisting of a gel filtration step in 1 M acetic acid followed by chromatography of the active pool on carboxymethylcellulose at pH 5. The molecular weight of the native protein was found to be 28000, and this value was reduced by approximately one-half under denaturing conditions. These values are comparable to those obtained for mouse 2.5S or betaNGF. Tryptic peptide maps of S-[14C]carboxymethyl NGF gave the number of labeled peptides expected for a structure composed of two identical or very similar subunits. Thus, the quaternary structures of mouse and cobra NGF are the same. Cyanogen bromide (CNBr) treatment of Naja naja NGF produced three fragments, of which two were purified to homogeneity. These fragments and the whole protein were analyzed in the automated protein Sequencer. The amino-terminal CNBr fragment of the protein was also subjected to digestion by thermolysin and the resultant peptides were purified and characterized. These data plus those from the characterization of the tryptic peptides provided the basis of the construction of a tentative primary structure of Naja naja NGF which is approximately 60% identical with mouse NGF.
肾上腺切除术诱导的大脑生长的位点特异性。
影响因子：
5.3
作者：
Thomas,TL;Devenport,LD
通讯作者：
Devenport,LD