Reliability and sensitivity of the TonoLab rebound tonometer in awake Brown Norway rats.
Reliability and sensitivity of the TonoLab rebound tonometer in awake Brown Norway rats.
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DOI：
10.1167/iovs.08-2465
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发表时间：
2009-06
影响因子：
4.4
通讯作者：
Johnson E
中科院分区：
文献类型：
--
作者：
Morrison JC;Jia L;Cepurna W;Guo Y;Johnson E
在清醒的Brown Norway大鼠中比较TonoLab回弹眼压计与Tono-Pen的灵敏度，并比较其预测实验性IOP升高诱导的视神经损伤的能力。TonoLab和Tono-Pen眼压计在连接到压力传感器的插管大鼠眼中进行校准。TonoLab用于饲养在标准照明下的清醒动物，以测量亮相和暗相期间的IOP。两种仪器都用于监测由巩膜外静脉注射高渗盐水引起的慢性升高的IOP。测量的IOP与注射眼的定量视神经损伤相关。尽管在所有水平上均低于传感器和Tono-Pen测量值，但TonoLab读数显示出与插管眼中20至80 mm Hg（R2 = 0.99）的传感器读数的良好线性拟合。在标准照明条件下饲养的清醒动物中，TonoLab记录到黑暗阶段（27.9 ± 1.7 mm Hg）的压力显著高于光照阶段（16.7 ± 2.3 mm Hg）。随着IOP升高，TonoLab和Tono-Pen读数之间的相关性（R2 = 0.86，P < 0.0001）与插管眼相似。虽然两种仪器提供的测量结果与视神经损伤等级相关性良好，但只有Tono-Pen记录了损伤程度最轻的眼睛的显著IOP升高（P < 0.05）。TonoLab的灵敏度足以用于清醒的Brown Norway大鼠，尽管仪器波动可能会限制其在最小视神经损伤的情况下识别眼睛中显著压力升高的能力。
To compare the sensitivity of the TonoLab rebound tonometer with the Tono-Pen in awake Brown Norway rats and to compare their ability to predict optic nerve damage induced by experimental IOP elevation. TonoLab and Tono-Pen tonometers were calibrated in cannulated rat eyes connected to a pressure transducer. The TonoLab was used in awake animals housed in standard lighting to measure IOP during light and dark phases. Both instruments were used to monitor chronically elevated IOP produced by episcleral vein injection of hypertonic saline. Measured IOPs were correlated with quantified optic nerve damage in injected eyes. Although they were lower than transducer and Tono-Pen measurements at all levels, TonoLab readings showed an excellent linear fit with transducer readings from 20 to 80 mm Hg (R2 = 0.99) in cannulated eyes. In awake animals housed in standard lighting, the TonoLab documented significantly higher pressures during the dark phase (27.9 ± 1.7 mm Hg) than during the light phase (16.7 ± 2.3 mm Hg). With elevated IOP, correlation between TonoLab and Tono-Pen readings (R2 = 0.86, P < 0.0001) was similar to that in cannulated eyes. Although both instruments provided measurements that correlated well with optic nerve injury grade, only the Tono-Pen documented significant IOP elevation in eyes with the least amount of injury (P < 0.05). The TonoLab is sensitive enough to be used in awake Brown Norway rats, though instrument fluctuation may limit its ability to identify significant pressure elevations in eyes with minimal optic nerve damage.
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影响因子：
3.4
作者：
Dalke, C;Pleyer, U;Graw, J
通讯作者：
Graw, J
影响因子：
3.4
作者：
Grozdanic, SD;Betts, DM;Sonea, IM
通讯作者：
Sonea, IM
影响因子：
3.4
作者：
Johnson, Elaine C.;Cepurna, William O.;Morrison, John C.
通讯作者：
Morrison, John C.
影响因子：
4.4
作者：
Johnson, Elaine C.;Jia, Lijun;Morrison, John C.
通讯作者：
Morrison, John C.
影响因子：
3.4
作者：
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通讯作者：
Mittag, T