Bone Regeneration With a Collagen Model Polypeptides/&agr;-Tricalcium Phosphate Sponge in a Canine Tibia Defect Model
Bone Regeneration With a Collagen Model Polypeptides/&agr;-Tricalcium Phosphate Sponge in a Canine Tibia Defect Model
复制标题
用胶原蛋白模型多肽进行骨再生/
DOI：
10.1097/id.0000000000000210
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发表时间：
2015
影响因子：
--
通讯作者：
S. Morita
中科院分区：
文献类型：
--
作者：
Tomohiko Ito;Y. Hashimoto;S. Baba;T. Iseki;S. Morita
引言：我们评估了由脯氨酸 - 羟脯氨酸 - 甘氨酸（聚（PHG））序列组成的合成胶原蛋白模型多肽与多孔α - 磷酸三钙（α - TCP）颗粒联合应用对犬胫骨缺损模型中骨形成的影响。
材料与方法：将多孔α - TCP颗粒与聚（PHG）溶液混合，然后对所得海绵进行交联，并通过X射线衍射和扫描电子显微镜进行表征。使用微型计算机断层扫描和组织学评估对12只健康比格犬的胫骨缺损进行分析。
结果：在第2周和第4周时，聚（PHG）/α - TCP组的新骨体积密度高于单独聚（PHG）组（P < 0.05）；然而，在第8周时无差异（P > 0.05）。植入后4周的组织学评估显示聚（PHG）已降解，并且在α - TCP颗粒表面有新形成的骨。在第8周时，两组的骨缺损顶部均有具有哈弗斯系统结构的连续皮质骨形成。
结论：本研究表明，使用多孔α - TCP颗粒和聚（PHG）制成的复合材料足以适用于治疗骨缺损。
Introduction:We evaluated the effects of synthesized collagen model polypeptides consisting of a proline-hydroxyproline-glycine (poly(PHG)) sequence combined with porous alpha-tricalcium phosphate (&agr;-TCP) particles on bone formation in a canine tibia defect model. Materials and Methods:The porous &agr;-TCP particles were mixed with a poly(PHG) solution, and the obtained sponge was then cross-linked and characterized by x-ray diffraction and scanning electron microscopy. Tibia defects were analyzed in 12 healthy beagles using microcomputed tomography and histological evaluation. Results:At 2 and 4 weeks, the volume density of new bone was higher in the poly(PHG)/&agr;-TCP group than in poly(PHG) alone group (P < 0.05); however, there was no difference at 8 weeks (P > 0.05). Histological evaluation at 4 weeks after implantation revealed that the poly(PHG) had degraded, and newly formed bone was present on the surface of the &agr;-TCP particles. At 8 weeks, continuous cortical bone formation with a Haversian structure covered the top of the bone defects in both groups. Conclusion:This study demonstrates that the composite created using porous &agr;-TCP particles and poly(PHG) is sufficiently adaptable for treating bone defects.
DOI：
10.1002/jbm.b.30540
发表时间：
2006-11
期刊：
Journal of biomedical materials research. Part B, Applied biomaterials
影响因子：
--
作者：
Hidemichi Kihara;M. Shiota;Y. Yamashita;S. Kasugai
通讯作者：
Hidemichi Kihara;M. Shiota;Y. Yamashita;S. Kasugai
用于骨再生的β-磷酸三钙/胶原海绵复合材料的开发。
DOI：
--
发表时间：
2006
期刊：
Dental Materials Journal 25
影响因子：
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作者：
Matsuno T;Nakamura T;et al.
通讯作者：
et al.