Protein transfer from isoelectric focusing Gels: the native blot.
Protein transfer from isoelectric focusing Gels: the native blot.
复制标题
等电聚焦凝胶的蛋白质转移:天然印迹。
DOI:
10.1016/0003-2697(82)90439-0
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发表时间:
1982
影响因子:
2.9
通讯作者:
Malamud,D
中科院分区:
文献类型:
--
作者:
Reinhart,MP;Malamud,D
The nonelectrophoretic transfer of proteins from thin (0.5 mm) isoelectric focusing gels to nitrocellulose was complete in 1 h. Blotting was bidirectional with 60% of the protein transferred to the top blot and the remaining 40% to the bottom blot. The use of nondenaturing transfer buffers permits proteins to be blotted in the native state. Immunological determinants are preserved and enzyme activity can be detected on the blots.
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影响因子:
14.9
作者:
Shen,V;King,TC;Kumar,V;Daugherty,B
通讯作者:
Daugherty,B
DOI:
10.5555/uri:pii:0022214353901267
发表时间:
1953
期刊:
The Journal of laboratory and clinical medicine
影响因子:
--
作者:
W. A. Curby
通讯作者:
W. A. Curby
影响因子:
14.9
作者:
Stellwag,EJ;Dahlberg,AE
通讯作者:
Dahlberg,AE
DOI:
10.1016/0005-2787(78)90125-9
发表时间:
1978
期刊:
Biochimica et biophysica acta
影响因子:
--
作者:
J. M. Glick;V. M. Averyhart;P. Leboy
通讯作者:
P. Leboy